Single human oocyte transcriptome analysis reveals distinct maturation stage-dependent pathways impacted by age.
Single human oocyte transcriptome analysis reveals distinct maturation stage-dependent pathways impacted by age.
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DOI：
10.1111/acel.13360
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发表时间：
2021-05
期刊：
影响因子：
7.8
通讯作者：
Payer B
中科院分区：
文献类型：
--
作者：
Llonch S;Barragán M;Nieto P;Mallol A;Elosua-Bayes M;Lorden P;Ruiz S;Zambelli F;Heyn H;Vassena R;Payer B
Female fertility is inversely correlated with maternal age due to a depletion of the oocyte pool and a reduction in oocyte developmental competence. Few studies have addressed the effect of maternal age on the human mature oocyte (MII) transcriptome, which is established during oocyte growth and maturation, however, the pathways involved remain unclear. Here, we characterize and compare the transcriptomes of a large cohort of fully grown germinal vesicle stage (GV) and in vitro matured (IVM‐MII) oocytes from women of varying reproductive age. First, we identified two clusters of cells reflecting the oocyte maturation stage (GV and IVM‐MII) with 4445 and 324 putative marker genes, respectively. Furthermore, we identified genes for which transcript representation either progressively increased or decreased with age. Our results indicate that the transcriptome is more affected by age in IVM‐MII oocytes (1219 genes) than in GV oocytes (596 genes). In particular, we found that transcripts of genes involved in chromosome segregation and RNA splicing significantly increased representation with age, while genes related to mitochondrial activity showed a lower representation. Gene regulatory network analysis facilitated the identification of potential upstream master regulators of the genes involved in those biological functions. Our analysis suggests that advanced maternal age does not globally affect the oocyte transcriptome at GV or IVM‐MII stages. Nonetheless, hundreds of genes displayed altered transcript representation, particularly in IVM‐MII oocytes, which might contribute to the age‐related quality decline in human oocytes. Here we used single‐oocyte RNA‐Seq to study the transcriptome changes occurring during human oocyte aging. We sampled Germinal Vesicle (GV) stage oocytes from women of age 18–43 and analyzed them directly (GV) or after in vitro maturation (IVM‐MII). We observed that age has a bigger impact on the IVM‐MII transcriptome. Transcripts belonging to biological pathways critical for oocyte maturation and function either increased (green, top) or decreased (red, bottom) in representation with age.
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影响因子：
3.6
作者：
Liguori I;Russo G;Curcio F;Bulli G;Aran L;Della-Morte D;Gargiulo G;Testa G;Cacciatore F;Bonaduce D;Abete P
通讯作者：
Abete P
DOI：
10.1016/j.cub.2015.12.073
发表时间：
2016-03-07
期刊：
Current biology : CB
影响因子：
--
作者：
Burkhardt S;Borsos M;Szydlowska A;Godwin J;Williams SA;Cohen PE;Hirota T;Saitou M;Tachibana-Konwalski K
通讯作者：
Tachibana-Konwalski K
影响因子：
12.3
作者：
Guillaumet-Adkins A;Rodríguez-Esteban G;Mereu E;Mendez-Lago M;Jaitin DA;Villanueva A;Vidal A;Martinez-Marti A;Felip E;Vivancos A;Keren-Shaul H;Heath S;Gut M;Amit I;Gut I;Heyn H
通讯作者：
Heyn H
影响因子：
4
作者：
Fragouli, E.;Bianchi, V.;Wells, D.
通讯作者：
Wells, D.
影响因子：
4.6
作者：
Ma, J;Zeng, FY;Tseng, H
通讯作者：
Tseng, H