Transmission electron microscopy of zebrafish spinal motor nerve roots.
Transmission electron microscopy of zebrafish spinal motor nerve roots.
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DOI：
10.1002/dvdy.24529
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Kucenas S
中科院分区：
文献类型：
--
作者：
Morris AD;Erisir A;Criswell SJ;Kucenas S
脊髓运动神经对于在中枢和外周神经系统之间传递信息是必不可少的。构成这些神经的细胞类型的扰动可能会损害动作电位的快速有效传递并改变神经功能。通过透射电子显微镜（TEM）识别这些细胞成分缺陷引起的超微结构变化可以为神经功能和疾病提供有价值的见解。然而，有效地定位脊髓运动神经在成年斑马鱼的TEM是具有挑战性的和耗时的。正因为如此，我们开发了一个协议，使我们能够快速，准确地定位脊髓运动神经根在成年斑马鱼的TEM处理。固定后，将从躯干区域解剖的成年斑马鱼的横向板安装在包埋介质中，切片并用四氧化锇进行二次固定。选择含有运动神经的横切面进行TEM断层扫描和成像。我们开发了一种有效的协议，定位脊髓运动神经在成年斑马鱼，允许超微结构特征。虽然我们的工作重点是脊髓运动神经，该协议可能是有用的，有效地确定其他离散的，重复的结构内的发展和成熟的神经系统，很难找到通过传统的，整个生物体TEM处理。
Spinal motor nerves are essential for relaying information between the central and peripheral nervous systems. Perturbations to cell types that compose these nerves may impair rapid and efficient transmission of action potentials and alter nerve function. Identifying ultrastructural changes resulting from defects to these cellular components via transmission electron microscopy (TEM) can provide valuable insight into nerve function and disease. However, efficiently locating spinal motor nerves in adult zebrafish for TEM is challenging and time consuming. Because of this, we developed a protocol that allows us to quickly and precisely locate spinal motor nerve roots in adult zebrafish for TEM processing. Following fixation, a transverse slab of adult zebrafish dissected from the trunk region was mounted in embedding media, sectioned and secondary fixation with osmium tetraoxide was performed. Transverse sections containing motor nerves were selected for TEM ultrathin sectioning and imaging. We developed an efficient protocol for locating spinal motor nerves in adult zebrafish to allow for ultrastructural characterization. Although our work focuses on spinal motor nerves, this protocol may be useful for efficiently identifying other discrete, repeated structures within the developing and mature nervous system that are difficult to find via traditional, whole organism TEM processing.
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DOI：
10.1126/science.1173474
发表时间：
2009-09-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Monk KR;Naylor SG;Glenn TD;Mercurio S;Perlin JR;Dominguez C;Moens CB;Talbot WS
通讯作者：
Talbot WS
影响因子：
2.1
作者：
Irwin, Jacob A.;Erisir, Alev;Kwon, Inchan
通讯作者：
Kwon, Inchan
影响因子：
2.7
作者：
Larson TA;Gordon TN;Lau HE;Parichy DM
通讯作者：
Parichy DM
影响因子：
3.3
作者：
Winey M;Meehl JB;O'Toole ET;Giddings TH Jr
通讯作者：
Giddings TH Jr
影响因子：
3.3
作者：
Corson, J.;Nahmani, M.;Lubarsky, K.;Badr, N.;Wright, C.;Erisir, A.
通讯作者：
Erisir, A.