Root Microbiota in Primary and Secondary Apical Periodontitis.
Root Microbiota in Primary and Secondary Apical Periodontitis.
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DOI：
10.3389/fmicb.2018.02374
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发表时间：
2018
影响因子：
5.2
通讯作者：
Lazarevic V
中科院分区：
文献类型：
--
作者：
Bouillaguet S;Manoil D;Girard M;Louis J;Gaïa N;Leo S;Schrenzel J;Lazarevic V
根尖牙周炎是一种由坏死根管内细菌定植引起的根周组织炎症性疾病。原发性根尖周炎是由坏死牙髓组织中的微生物定植引起的。继发性根尖周炎是由于根管治疗不当引起的持续性感染。本研究的目的是使用16 S rRNA基因扩增子测序来表征与根尖牙周炎相关的原发性和继发性根内感染中存在的微生物群。在知情同意的情况下，对表现出根尖周炎的牙齿进行根管治疗或未进行根管治疗。从每颗牙齿中，收集根内内容物以及牙本质样品（对照）并进行DNA提取。合并细菌16 S rRNA基因V3-V4区的PCR扩增子，并在Illumina MiSeq仪器上测序（2 × 300）。生物信息学分析管道包括质量过滤、正向和反向读段的合并、读段聚类成操作分类单元（OTU）、去除推定的污染物OTU和分配分类。牙本质和根管样本中最普遍和丰富的OTU被分配给厌氧细菌具核梭杆菌。多变量分析显示，根据样本类型（牙本质与根内内容物）以及根管中的病理学（原发性与继发性感染），微生物群聚类。粪肠球菌和F.继发感染根管与原发感染根管相比，核质含量分别较高和较低。共现网络分析提供了针对感染类型的微生物相互作用的证据。鉴别原发性和继发性根尖内感染中差异丰富的细菌类群可能为旨在降低根尖牙周炎发病率的靶向治疗方法提供基础。
Apical periodontitis is an inflammatory disease of the dental periradicular tissues triggered by bacteria colonizing necrotic root canals. Primary apical periodontitis results from the microbial colonization of necrotic pulp tissues. Secondary apical periodontitis results from a persistent infection of incorrectly treated root canals. The aim of this study was to characterize the microbiota present in primary and secondary intraradicular infections associated with apical periodontitis using 16S rRNA gene amplicon sequencing. Teeth exhibiting apical periodontitis with or without root canal treatment were extracted after informed consent. From each tooth, the intraradicular content as well as a dentin sample (control) were collected and subjected to DNA extraction. PCR amplicons of the V3–V4 region of the bacterial 16S rRNA gene were pooled and sequenced (2 × 300) on an Illumina MiSeq instrument. The bioinformatics analysis pipeline included quality filtering, merging of forward and reverse reads, clustering of reads into operational taxonomic units (OTUs), removal of putative contaminant OTUs and assigning taxonomy. The most prevalent and abundant OTU in both dentin and root canal samples was assigned to anaerobic bacterium Fusobacterium nucleatum. Multivariate analysis showed clustering of microbiota by sample type (dentin vs. intraradicular content) and, in root canals, by pathology (primary vs. secondary infection). The proportions of Enterococcus faecalis and F. nucleatum were, respectively, higher and lower when comparing secondary to primary infected root canals. Co-occurrence network analysis provided evidence of microbial interactions specific to the infection type. The identification of bacterial taxa differentially abundant in primary and secondary intraradicular infections may provide the basis for targeted therapeutic approaches aimed at reducing the incidence of apical periodontitis.
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影响因子：
4.3
作者：
Faust K;Sathirapongsasuti JF;Izard J;Segata N;Gevers D;Raes J;Huttenhower C
通讯作者：
Huttenhower C
影响因子：
4.8
作者：
Clark, JS
通讯作者：
Clark, JS
影响因子：
7.6
作者：
Caplan, D. J.;Chasen, J. B.;Beck, J. D.
通讯作者：
Beck, J. D.
影响因子：
5
作者：
ANDO, N;HOSHINO, E
通讯作者：
HOSHINO, E
影响因子：
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作者：
Mayanagi, G;Sato, T;Takahashi, N
通讯作者：
Takahashi, N