Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination.
Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination.
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DOI：
10.1093/nar/gkm823
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发表时间：
2007
影响因子：
14.9
通讯作者：
Lieber MR
中科院分区：
文献类型：
--
作者：
Lu H;Schwarz K;Lieber MR
V（D）J重组事件通过RAG1：RAG2复合物在基因片段处切割而启动，这导致在编码末端形成发夹。发夹由Artemis：DNA-PKcs复合物打开，然后通过非同源DNA末端连接（NHEJ）过程连接。在这里，我们研究了所有39个功能性人VH元件的发夹编码末端的开放。我们发现Artemis：DNA-PKcs的发夹打开效率甚至位置存在一些序列依赖性变化。发夹打开效率在7倍范围内变化。发夹的开口位置在发夹末端的1 - 4 nt 3 ′区域内变化，导致在每个编码末端处形成2 - 8 nt的单链3 ′突出端。该信息提供了关于发夹开口位置在V（D）J重组中对连接多样化的贡献程度的更大的清晰度。
V(D)J recombination events are initiated by cleavage at gene segments by the RAG1:RAG2 complex, which results in hairpin formation at the coding ends. The hairpins are opened by the Artemis:DNA-PKcs complex, and then joined via the nonhomologous DNA end joining (NHEJ) process. Here we examine the opening of the hairpinned coding ends from all of the 39 functional human VH elements. We find that there is some sequence-dependent variation in the efficiency and even the position of hairpin opening by Artemis:DNA-PKcs. The hairpin opening efficiency varies over a 7-fold range. The hairpin opening position varies over the region from 1 to 4 nt 3′ of the hairpin tip, leading to a 2–8 nt single-stranded 3′ overhang at each coding end. This information provides greater clarity on the extent to which the hairpin opening position contributes to junctional diversification in V(D)J recombination.
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影响因子：
4.8
作者：
Chan, DW;LeesMiller, SP
通讯作者：
LeesMiller, SP
影响因子：
64.5
作者：
MCBLANE, JF;VANGENT, DC;OETTINGER, MA
通讯作者：
OETTINGER, MA
影响因子：
14.9
作者：
Block, WD;Yu, YP;Lees-Miller, SP
通讯作者：
Lees-Miller, SP
影响因子：
5.3
作者：
Schlissel, MS
通讯作者：
Schlissel, MS
影响因子：
4.8
作者：
LIEBER, MR
通讯作者：
LIEBER, MR