Single-cell RNA-sequencing of retrieved human oocytes and eggs in clinical practice and for human ovarian cell atlasing.
Single-cell RNA-sequencing of retrieved human oocytes and eggs in clinical practice and for human ovarian cell atlasing.
复制标题
DOI：
10.1002/mrd.23648
复制
发表时间：
2022-12
影响因子：
2.5
通讯作者：
Shikanov, Ariella
中科院分区：
文献类型：
--
作者：
Machlin, Jordan H.;Shikanov, Ariella
随着单细胞分离技术和高通量测序平台的进步，单细胞RNA测序（scRNA - seq）已经成为在细胞分辨率上理解组织和器官系统的重要技术。通过转录分析，有可能表征独特或罕见的细胞类型，解释它们的相互作用，并揭示新的功能状态或发育阶段的转变。因此，这项技术特别适合于研究人类卵巢内的细胞。卵巢是一个细胞异质性的器官，它容纳卵泡、生殖和内分泌单位，包括被产生激素的支持细胞包围的卵母细胞，以及构成基质、脉管系统、淋巴和免疫成分的许多其他细胞群。在此，我们回顾了利用scRNA - seq技术分析健康人类卵巢细胞的研究，并讨论了所使用的单细胞分离技术。我们确定了scRNA - seq在人类卵巢中的两个主要应用。第一个应用这项技术来研究接受体外受精治疗的患者卵母细胞/卵子的转录差异，以最终改善临床结果。第二种方法是利用scRNA - seq技术建立人类卵巢的全面单细胞图谱。从这些研究中获得的知识强调了scRNA - seq技术在解开人类卵巢生物学新认识方面的重要性。
With the advancement of single‐cell separation techniques and high‐throughput sequencing platforms, single‐cell RNA‐sequencing (scRNA‐seq) has emerged as a vital technology for understanding tissue and organ systems at cellular resolution. Through transcriptional analysis, it is possible to characterize unique or rare cell types, interpret their interactions, and reveal novel functional states or shifts in developmental stages. As such, this technology is uniquely suited for studying the cells within the human ovary. The ovary is a cellularly heterogeneous organ that houses follicles, the reproductive and endocrine unit that consists of an oocyte surrounded by hormone‐producing support cells, as well as many other cell populations constituting stroma, vasculature, lymphatic, and immune components. Here we review studies that have utilized scRNA‐seq technology to analyze cells from healthy human ovaries and discuss the single‐cell isolation techniques used. We identified two overarching applications for scRNA‐seq in the human ovary. The first applies this technology to investigate transcriptional differences in oocytes/eggs from patients undergoing in vitro fertilization treatments to ultimately improve clinical outcomes. The second utilizes scRNA‐seq for the pursuit of creating a comprehensive single‐cell atlas of the human ovary. The knowledge gained from these studies underscores the importance of scRNA‐seq technologies in unlocking a new biological understanding of the human ovary.
登录
查看更多内容
影响因子：
2.5
作者：
Amini P;Ramezanali F;Parchehbaf-Kashani M;Maroufizadeh S;Omani-Samani R;Ghaheri A
通讯作者：
Ghaheri A
影响因子：
5.6
作者：
Fan X;Moustakas I;Bialecka M;Del Valle JS;Overeem AW;Louwe LA;Pilgram GSK;van der Westerlaken LAJ;Mei H;Chuva de Sousa Lopes SM
通讯作者：
Chuva de Sousa Lopes SM
影响因子：
4.7
作者：
Kanter I;Kalisky T
通讯作者：
Kalisky T
影响因子：
6.1
作者：
De Vos, A;Van de Velde, H;Van Steirteghem, A
通讯作者：
Van Steirteghem, A
DOI：
10.1016/j.ogc.2014.09.005
发表时间：
2015-03-01
影响因子：
3.2
作者：
Crawford, Natalie M.;Steiner, Anne Z.
通讯作者：
Steiner, Anne Z.