Molecular cloning and characterization of p38 gene in the Chinese Mitten Crab, Eriocheir sinensis
Molecular cloning and characterization of p38 gene in the Chinese Mitten Crab, Eriocheir sinensis
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中华绒螯蟹p38基因的分子克隆及鉴定
DOI：
10.1111/are.12590
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发表时间：
2016-04
影响因子：
2
通讯作者：
He Lin
中科院分区：
文献类型：
--
作者：
Zhao Jin Cheng;Wang Yuan Li;Li Qing;Zhu Ming;Sun Wen Juan;Wu Tian Min;Wang Qun;He Lin
P38是丝氨酸/苏氨酸蛋白激酶，是MAPK信号通路的重要组成部分。我们克隆了中华绒螯蟹p38(Es-p38)全长cDNA序列，该序列包含1098个核苷酸的ORF，编码一个365个氨基酸的蛋白质。ES-p38蛋白具有p38MAPK功能区(STKc_P38)，是一种典型的丝氨酸/苏氨酸激酶，处理所有p38蛋白保守的T-G-Y基序。用实时荧光定量聚合酶链式反应技术检测ES-p38基因在组织中的表达及在睾丸发育阶段的表达。结果表明，ES-p38基因在不同组织中均有表达，但在肌肉、肝胰腺、肠、卵巢和睾丸中表达较高。ES-p38基因在睾丸发育的不同阶段的表达水平在精母细胞期(7-8月)和精子细胞期(8-10月)之间无显著差异，但在精子期的中晚期(12-1月)显著升高。这一变化趋势与中华绒毛虫的性腺发育和生殖阶段呈正相关，初步表明ES-p38可能参与中华绒毛虫的睾丸发育和精子发生过程。
p38 is an important component of the MAPK signaling pathways as a Ser/Thr protein kinase. We cloned the full length p38 (Es-p38) cDNA sequence in Eriocheir Sinensis which contained a 1098nucleotide ORF that encoded a protein of 365 amino acids. Es-p38 protein has a p38 MAPK functional domain (STKc_p38), it is a typical serine/threonine kinase and processes all p38 protein’s conservative T-G-Y motif. Tissue expression of the Es-p38 gene and expression during testis development stages were determined by quantitative real-time PCR analysis. The results show that Es-p38 gene is expressed in various tissues, but it expresses higher in the muscle, hepatopancreas, intestine, ovary and testis. The expression level of Es-p38 gene at different stages of testis development shows no significant difference between the spermatocyte phase (July–August) and spermatid stage (August–October), but it rises significantly in the mid-late period of sperm stage (December– January). This changing trend has a positive relationship with the E. Sinensis gonad development and reproduction stage, which preliminarily shows that Es-p38 may function in the E. Sinensis testis development and the process of spermatogenesis.
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DOI：
--
发表时间：
2003
期刊：
Journal of the Fourth Military Medical University
影响因子：
--
作者：
Jiang Er
通讯作者：
Jiang Er
影响因子：
13.6
作者：
Li MW;Mruk DD;Cheng CY
通讯作者：
Cheng CY
影响因子：
4.1
作者：
Almog, Tal;Naor, Zvi
通讯作者：
Naor, Zvi
影响因子：
56.9
作者：
HAN, J;LEE, JD;ULEVITCH, RJ
通讯作者：
ULEVITCH, RJ
影响因子：
3.8
作者：
Liang Zhang;F. Meng;Yuzhen Li;M. Kang;Xingqi Guo;Baohua Xu
通讯作者：
Liang Zhang;F. Meng;Yuzhen Li;M. Kang;Xingqi Guo;Baohua Xu