Differential expression of human HOX-2 genes along the anterior-posterior axis in embryonic central nervous system.
Differential expression of human HOX-2 genes along the anterior-posterior axis in embryonic central nervous system.
复制标题
胚胎中枢神经系统中人类 HOX-2 基因沿前后轴的差异表达。
DOI：
10.1111/j.1432-0436.1989.tb00598.x
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
C. Peschle
中科院分区：
文献类型：
--
作者：
A. Giampaolo;D. Acampora;V. Zappavigna;M. Pannese;M. D'Esposito;A. Carè;Antonio Feiella;A. Stornaiuolo;G. Russo;A. Simeone;E. Boncinelli;C. Peschle
我们研究了人类HOX-2基因座的结构，该基因座包含染色体17 q21上的90 kb区域。5个新的人类HOX-2同源框，命名为HOX-2.5，2.4，2.6，2.7和2.8，已被确定，并报告其核苷酸序列。它们具有相同的5 '-3'转录方向，并且与三个先前描述的HOX-2同源框（5 '-2.5-2.4-2.3-2.2-2.1-2.6-2.7-2.8- 3'）成簇。我们还研究了HOX-2基因的区域特异性表达的人胚胎-胎儿的生活通过Northern-blot分析。所有的基因都在整个胚胎和怀孕5-9周的胎儿中表达。它们的主要表达位点位于中枢神经系统（CNS）内，尽管它们在CNS以外的身体结构中以较低水平转录。它们在CNS中相对丰富的表达已经通过解剖脑、延髓和脊髓本身沿沿着前后轴进行了分析。HOX-2.5、2.4和2.3转录物在脊髓中比在髓质中明显更丰富，而2.2、2.1、2.6和2.7 mRNA在髓质中逐渐更丰富。此外，检测到HOX-2.1、2.6、2.7、2.8在脑中的表达，尽管水平较低。因此，HOX-2同源框基因沿着染色体5 '-3'方向的相对位置似乎与它们的表达结构域沿沿着CNS纵轴尾-头方向的相对位置相关。
We have investigated the structure of the human HOX-2 locus, which encompasses a 90-kb region on chromosome 17q21. Five new human HOX-2 homeoboxes, termed HOX-2.5, 2.4, 2.6, 2.7 and 2.8, have been identified, and their nucleotide sequences are reported. They have the same 5'-3' transcriptional orientation and are clustered with three previously described HOX-2 homeoboxes (5'-2.5-2.4-2.3-2.2-2.1-2.6-2.7-2.8-3'). We have also investigated the region-specific expression of HOX-2 genes in human embryonic-fetal life by Northern-blot analysis. All genes are expressed in whole embryos and fetuses at 5-9 weeks from conception. Their major site of expression lies within the central nervous system (CNS), although they are transcribed at a lower level in body structures other than the CNS. Their relatively abundant expression in CNS has been analyzed along the anterior-posterior axis by dissecting the brain, the medulla oblongata and the spinal cord proper. HOX-2.5, 2.4 and 2.3 transcripts are markedly more abundant in spinal cord than in medulla, whereas 2.2, 2.1, 2.6 and 2.7 mRNAs are progressively more abundant in the medulla. Additionally, expression in brain was detected, although at lower level, for HOX-2.1, 2.6, 2.7, 2.8. Thus, the relative position of HOX-2 homeobox genes along the chromosome in the 5'-3' direction appears to correlate with the relative position of their expression domains along the CNS longitudinal axis in the caudal-cephalic direction.
DOI：
10.1073/pnas.81.13.4115
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
SCOTT, MP;WEINER, AJ
通讯作者：
WEINER, AJ
Hox-2.2（一种小鼠同源框基因）的结构和表达。
DOI：
10.1073/pnas.85.15.5582
发表时间：
1988
影响因子：
11.1
作者：
Schughart,K;Utset,MF;Awgulewitsch,A;Ruddle,FH
通讯作者：
Ruddle,FH
鼠类蛋白的同源结构域与其自身的同源框结合 5。
DOI：
10.1073/pnas.83.24.9532
发表时间：
1986
影响因子：
11.1
作者：
Fainsod,A;Bogarad,LD;Ruusala,T;Lubin,M;Crothers,DM;Ruddle,FH
通讯作者：
Ruddle,FH