Identification and characterization of serine protease inhibitors in a parasitic wasp, Pteromalus puparum.
Identification and characterization of serine protease inhibitors in a parasitic wasp, Pteromalus puparum.
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寄生蜂 Pteromalus puparum 中丝氨酸蛋白酶抑制剂的鉴定和表征
DOI：
10.1038/s41598-017-16000-5
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发表时间：
2017-11-16
影响因子：
4.6
通讯作者：
Ye G
中科院分区：
文献类型：
--
作者：
Yang L;Mei Y;Fang Q;Wang J;Yan Z;Song Q;Lin Z;Ye G
丝氨酸蛋白酶抑制剂（SPI）通过灭活其同源蛋白酶来调节蛋白酶介导的活性，并参与多种生理过程。SPIs在脊椎动物和无脊椎动物中得到了广泛的研究，然而，在寄生虫中的SPI信息很少。在此，我们通过寄生蜂蛹金小蜂的基因组，共鉴定了57个SPI基因。基因结构分析表明，这些SPI含有7个SPI结构域。根据它们的作用模式，这些SPI可以分为丝氨酸蛋白酶抑制剂、典型抑制剂和α-2-巨球蛋白（A2 M）。对于丝氨酸蛋白酶抑制剂和典型的抑制剂，我们预测其推定的抑制活性胰蛋白酶/胰凝乳蛋白酶/弹性蛋白酶样酶的基础上切割的反应位点的氨基酸。序列比对和系统进化树表明，一些与已知抑制性丝氨酸蛋白酶抑制剂功能相似的丝氨酸蛋白酶抑制剂可能参与免疫应答。转录组分析还显示，一些典型的SPI基因在毒腺中显示出不同的表达模式，这被定量实时PCR（qPCR）分析所证实，表明它们作为毒液蛋白在抑制宿主免疫反应中具有特定的生理功能。该研究为阐明SPI在消化、发育、生殖和天然免疫中的功能提供了有价值的信息。
Serine protease inhibitors (SPIs) regulate protease-mediated activities by inactivating their cognate proteinases, and are involved in multiple physiological processes. SPIs have been extensively studied in vertebrates and invertebrates; however, little SPI information is available in parasitoids. Herein, we identified 57 SPI genes in total through the genome of a parasitoid wasp, Pteromalus puparum. Gene structure analyses revealed that these SPIs contain 7 SPI domains. Depending on their mode of action, these SPIs can be categorized into serpins, canonical inhibitors and alpha-2-macroglobulins (A2Ms). For serpins and canonical inhibitors, we predicted their putative inhibitory activities to trypsin/chymotrypsin/elastase-like enzymes based on the amino acids in cleaved reactive sites. Sequence alignment and phylogenetic tree indicated that some serpins similar to known functional inhibitory serpins may participate in immune responses. Transcriptome analysis also showed some canonical SPI genes displayed distinct expression patterns in the venom gland and this was confirmed by quantitative real-time PCR (qPCR) analysis, suggesting their specific physiological functions as venom proteins in suppressing host immune responses. The study provides valuable information to clarify the functions of SPIs in digestion, development, reproduction and innate immunity.
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影响因子：
4
作者：
Ibelli AM;Kim TK;Hill CC;Lewis LA;Bakshi M;Miller S;Porter L;Mulenga A
通讯作者：
Mulenga A
影响因子：
2.5
作者：
Kramerova, IA;Kramerov, AA;Fessler, JH
通讯作者：
Fessler, JH
影响因子：
3.4
作者：
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通讯作者：
Huang JF
影响因子：
3.8
作者：
COLEMAN, S;DRAHN, B;KRAUS, K
通讯作者：
KRAUS, K
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影响因子：
3.8
作者：
Chu, Yuan;Zhou, Fan;An, Chunju
通讯作者：
An, Chunju