Molecular epidemiology of Japanese encephalitis in northern Vietnam, 1964-2011: genotype replacement.
Molecular epidemiology of Japanese encephalitis in northern Vietnam, 1964-2011: genotype replacement.
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DOI：
10.1186/s12985-015-0278-4
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发表时间：
2015-04-01
期刊：
影响因子：
4.8
通讯作者：
Phan NT
中科院分区：
文献类型：
--
作者：
Do LP;Bui TM;Hasebe F;Morita K;Phan NT
日本脑炎病毒（JEV）是一种节肢动物传播的病毒，在亚洲引起严重的公共卫生问题。JEV由五种基因型组成，最近的研究表明JEV基因型I（GI）的出现及其对基因型III（GIII）的替代。使用JEV档案收集，我们在全球范围内调查了过去48年（1964-2012）越南人，蚊子和猪中JEV的分子进化。本研究中从人、猪和蚊子中分离的9株JEV和GenBank中可获得的29个序列用于分析越南JEV的包膜（E）蛋白。收集了225例疑似乙脑患者的脑脊液标本，用实时RT-PCR进行检测和基因分型。38个E基因测序和9个越南JEV株基因分型的实时RT-PCR，属于两个谱系，按照在世界其他地区的进化。1964年在越南的人类和1979年在蚊子中发现了第一个GIII菌株，而GI菌株分别于1990年和1994年首次在人类和蚊子中发现。2004年后，GI是越南检测到的唯一基因型，表明GI III菌株已被GI菌株取代。在越南JEV中鉴定出5种单倍型，以SKSS为主。E蛋白中的S123 N和S123 R替换已经存在于越南JEV中。这项研究描述了JEV在越南超过34年的漫长演变历史，这与JEV的全球演变密切相关。在蚊子、猪和人类中，越南的GIII菌株已被GI菌株取代。越南菌株的主要单倍型支持这种基因型置换在越南。需要进一步的监测，以确认在越南长期使用乙脑疫苗后，GIII菌株在自然界中消失，并出现新的脑炎病原体。本文的在线版本（doi：10.1186/s12985-015-0278-4）包含补充材料，可供授权用户使用。
Japanese encephalitis virus (JEV) is an arthropod-borne virus causing serious public health issues in Asia. JEV consists of five genotypes and recent studies have shown the emergence of JEV genotype I (GI) and its replacement of genotype III (GIII). Using an archival JEV collection, we investigated the molecular evolution of JEV in Vietnam over the last 48 years (1964–2012) in humans, mosquitoes, and pigs, within the global context. The nine JEV isolates from humans, pigs, and mosquitoes sequenced in this study and 29 sequences available in GenBank were used to analyze the envelope (E) protein of the Vietnamese JEVs. A collection of 225 cerebrospinal fluid specimens from patients with suspected Japanese encephalitis (JE) was also tested and genotyped with real-time RT–PCR. The 38 E genes identified with sequencing and nine Vietnamese JEV strains genotyped with real-time RT–PCR, belonging to two lineages, evolved in accordance with those in the rest of the world. The first GIII strain was detected in humans in Vietnam in 1964, and in mosquitoes in 1979, whereas GI strains were first detected in humans and mosquitoes in 1990 and 1994, respectively. After 2004, GI was the only genotype detected in Vietnam, demonstrating that the GIIII strains had been displaced by GI strains. Five haplotypes were identified in the Vietnamese JEVs, with SKSS predominant. The S123N and S123R substitutions in the E protein were already present in the Vietnamese JEVs. This study describes the long evolutionary history of JEV in Vietnam over 34 years, which correlates well with the global evolution of JEV. The Vietnamese GIII strains have been replaced by GI strains in mosquitoes, pigs, and humans. The predominant haplotypes of the Vietnamese strains support this genotype displacement in Vietnam. Further surveillance is required to confirm the disappearance of the GIII strains in nature and the emergence of new pathogens causing encephalitis in Vietnam, after the long-term use of JEV vaccines in that country. The online version of this article (doi:10.1186/s12985-015-0278-4) contains supplementary material, which is available to authorized users.
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发表时间：
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