Quantitative genome-wide enhancer activity maps for five Drosophila species show functional enhancer conservation and turnover during cis-regulatory evolution.
Quantitative genome-wide enhancer activity maps for five Drosophila species show functional enhancer conservation and turnover during cis-regulatory evolution.
复制标题
DOI：
10.1038/ng.3009
复制
发表时间：
2014-07
期刊：
影响因子：
30.8
通讯作者：
Stark A
中科院分区：
文献类型：
--
作者：
Arnold CD;Gerlach D;Spies D;Matts JA;Sytnikova YA;Pagani M;Lau NC;Stark A
Phenotypic differences between closely related species are thought to arise primarily from changes in gene expression due to mutations in cis-regulatory sequences (enhancers). However, it has remained unclear how frequently mutations alter enhancer activity or create functional enhancers de novo. Here we use STARR-seq, a recently developed quantitative enhancer assay, to determine genome-wide enhancer activity profiles for five Drosophila species in the constant trans-regulatory environment of Drosophila melanogaster S2 cells. We find that the functions of a large fraction of D. melanogaster enhancers are conserved for their orthologous sequences owing to selection and stabilizing turnover of transcription factor motifs. Moreover, hundreds of enhancers have been gained since the D. melanogaster–Drosophila yakuba split about 11 million years ago without apparent adaptive selection and can contribute to changes in gene expression in vivo. Our finding that enhancer activity is often deeply conserved and frequently gained provides functional insights into regulatory evolution.
登录
查看更多内容
影响因子：
30.8
作者：
He, Qiye;Bardet, Anais F.;Zeitlinger, Julia
通讯作者：
Zeitlinger, Julia
影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
影响因子：
4.5
作者：
Hare, Emily E.;Peterson, Brant K.;Iyer, Venky N.;Meier, Rudolf;Eisen, Michael B.
通讯作者：
Eisen, Michael B.
影响因子：
4.5
作者：
Lawrie DS;Messer PW;Hershberg R;Petrov DA
通讯作者：
Petrov DA
影响因子：
9.8
作者：
Ludwig MZ;Palsson A;Alekseeva E;Bergman CM;Nathan J;Kreitman M
通讯作者：
Kreitman M