Photobiomodulation does not influence maturation and mildly improves functional healing of mouse achilles tendons.
Photobiomodulation does not influence maturation and mildly improves functional healing of mouse achilles tendons.
复制标题
DOI：
10.1002/jor.24592
复制
发表时间：
2020-08
期刊：
影响因子：
--
通讯作者：
Killian ML
中科院分区：
文献类型：
--
作者：
Locke RC;Lemmon EA;Dudzinski E;Kopa SC;Wayne JM;Soulas JM;De Taboada L;Killian ML
肌腱断裂可以发生在任何年龄，通常采用非手术治疗，但可能导致持续的症状。因此，需要改进受伤肌腱的非手术治疗。光生物调节（PBM）疗法已在临床上显示出前景，并被假设为刺激尿道相关的代谢和改善愈合。然而，PBM治疗对肌腱成熟和愈合过程中线粒体功能的影响尚不清楚，其对肌腱结构和功能的影响仍不清楚。在这项研究中，近红外光（980：810 nm混合，2.5 J/cm 2）施加在低（30 mW/cm 2）或高（300 mW/cm 2）辐照CD-1小鼠的单侧跟腱在出生后的生长（成熟），以及成年小鼠双侧跟腱切开术（愈合）。PBM治疗对肌腱结构和功能的慢性影响通过组织学和力学来确定，PBM治疗对肌腱相关基因表达的急性影响进行了评估。在成熟和愈合过程中，胶原排列、细胞数量和核形状不受慢性PBM治疗的影响。我们发现愈合期间PBM治疗对机械结局的性别依赖性影响（例如，PBM治疗的女性刚度和杨氏模量增加，PBM治疗的男性极限应力下的应变增加）。线粒体相关基因表达的影响不大PBM治疗的成熟和愈合的研究。这项研究是第一个在小鼠肌腱的生长和愈合过程中实施PBM治疗的研究。PBM治疗对小鼠肌腱产生边缘和性别依赖性影响。临床意义：PBM可能有利于肌腱愈合，因为功能重建改善而无不良反应。
Tendon rupture can occur at any age and is commonly treated nonoperatively, yet can result in persisting symptoms. Thus, a need exists to improve nonoperative treatments of injured tendons. Photobiomodulation (PBM) therapy has shown promise in the clinic and is hypothesized to stimulate mitochondrial-related metabolism and improve healing. However, the effect of PBM therapy on mitochondrial function during tendon maturation and healing are unknown, and its effect on tendon structure and function remain unclear. In this study, near-infrared light (980:810 nm blend, 2.5 J/cm2) was applied at low (30 mW/cm2) or high (300 mW/cm2) irradiance to unilateral Achilles tendons of CD-1 mice during postnatal growth (maturation) as well as adult mice with bilateral Achilles tenotomy (healing). The chronic effect of PBM therapy on tendon structure and function was determined using histology and mechanics, and the acute effect of PBM therapy on mitochondrial-related gene expression was assessed. During maturation and healing, collagen alignment, cell number, and nuclear shape were unaffected by chronic PBM therapy. We found a sex-dependent effect of PBM therapy during healing on mechanical outcomes (eg, increased stiffness and Young's modulus for PBM-treated females, and increased strain at ultimate stress for PBM-treated males). Mitochondria-related gene expression was marginally influenced by PBM therapy for both maturation and healing studies. This study was the first to implement PBM therapy during both growth and healing of the murine tendon. PBM therapy resulted in marginal and sex-dependent effects on the murine tendon. Clinical significance: PBM may be beneficial for tendon healing because functional remodeling improves without adverse effects.
登录
查看更多内容
影响因子：
3.3
作者：
Hoffman NE;Chandramoorthy HC;Shanmughapriya S;Zhang XQ;Vallem S;Doonan PJ;Malliankaraman K;Guo S;Rajan S;Elrod JW;Koch WJ;Cheung JY;Madesh M
通讯作者：
Madesh M
影响因子：
1.3
作者：
Barfod, Kristoffer Weisskirchner;Sveen, Thor Magnus;Troelsen, Anders
通讯作者：
Troelsen, Anders
影响因子：
--
作者：
Iacopetti, Ilaria;Perazzi, Anna;Busetto, Roberto
通讯作者：
Busetto, Roberto
影响因子：
3.4
作者：
de Oliveira, Helenita Antonia;Antonio, Ednei Luiz;Serra, Andrey Jorge
通讯作者：
Serra, Andrey Jorge
影响因子：
--
作者：
Carrinho, Patricia M.;Muniz Renno, Ana Claudia;Vidal, Benedito Campos
通讯作者：
Vidal, Benedito Campos