Genome-Wide Characterization of DNA Methylation in an Invasive Lepidopteran Pest, the Cotton Bollworm Helicoverpa armigera.
Genome-Wide Characterization of DNA Methylation in an Invasive Lepidopteran Pest, the Cotton Bollworm Helicoverpa armigera.
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DOI：
10.1534/g3.117.1112
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发表时间：
2018-03-02
期刊：
影响因子：
--
通讯作者：
Bass C
中科院分区：
文献类型：
--
作者：
Jones CM;Lim KS;Chapman JW;Bass C
害虫的基因和基因组是由在其环境中遇到的广泛的选择力量形成的。虽然进化的分子适应开始在基因组和转录组水平上得到理解，但在表观遗传水平上却没有得到很好的表征。在这里，我们提出了棉铃虫蛾（Helicoverpa armigera）的DNA甲基化单核苷酸分辨率的全基因组图谱，这是一种全球入侵的农业害虫。我们发现，在棉蚜的幼虫和成虫中，甲基化几乎是相同的，通过全基因组亚硫酸盐测序（WGBS），该物种中最多0.9%的CpG位点被甲基化。我们发现DNA甲基化主要发生在外显子中，与基因表达呈正相关，并且甲基化的基因在细胞“管家”角色中富集。棉铃虫具有超常的远距离迁徙能力。为了探索甲基化在影响棉蚜迁徙表型中的作用，我们对从16个基因中选择的基因位点进行了靶向亚硫酸盐测序，这些基因在行为分析中表现出不同飞行表现的成年飞蛾之间表达差异。虽然这些基因中的大多数CpG位点在飞行表型之间没有甲基化，但我们在针对赖氨酸特异性组蛋白修饰的去甲基化酶（KDM4）中发现了高甲基化，该修饰与转录和甲基化密切相关。棉蚜甲基化组为夜蛾DNA甲基化的作用提供了新的见解，为进一步研究这种重要害虫的适应性性状的表观遗传控制提供了宝贵的资源。
The genes and genomes of insect pests are shaped by the wide array of selective forces encountered in their environments. While the molecular adaptations that evolve are beginning to be understood at the genomic and transcriptomic level, they have been less well characterized at an epigenetic level. Here, we present a genome-wide map of DNA methylation at single-nucleotide resolution for the cotton bollworm moth, Helicoverpa armigera, a globally invasive pest of agriculture. We show that methylation is almost identical in the larvae and adults of H. armigera and that, through whole-genome bisulfite sequencing (WGBS), at the most ∼0.9% of CpG sites in this species are methylated. We find that DNA methylation occurs primarily in exons, is positively correlated with gene expression, and that methylated genes are enriched for cellular “housekeeping” roles. H. armigera has an exceptional capacity for long-range migration. To explore the role of methylation in influencing the migratory phenotype of H. armigera, we performed targeted bisulfite sequencing on selected loci from 16 genes that were differentially expressed between adult moths exhibiting distinct flight performance in behavioral assays. While most CpG sites in these genes were not methylated between flight phenotypes, we identified hypermethylation in a demethylase (KDM4) that targets lysine-specific histone modifications, which are strongly associated with transcription and methylation. The H. armigera methylome provides new insights into the role of DNA methylation in a noctuid moth and is a valuable resource for further research into the epigenetic control of adaptive traits in this important pest.
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影响因子：
2.6
作者：
Hunt, Brendan G.;Glastad, Karl M.;Goodisman, Michael A. D.
通讯作者：
Goodisman, Michael A. D.
影响因子：
4.9
作者：
Jones CM;Papanicolaou A;Mironidis GK;Vontas J;Yang Y;Lim KS;Oakeshott JG;Bass C;Chapman JW
通讯作者：
Chapman JW
影响因子：
2.6
作者：
Glastad, Karl M.;Goodisman, Michael A. D.;Hunt, Brendan G.
通讯作者：
Hunt, Brendan G.
DOI：
10.1534/g3.113.008953
发表时间：
2014-03-20
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Beeler SM;Wong GT;Zheng JM;Bush EC;Remnant EJ;Oldroyd BP;Drewell RA
通讯作者：
Drewell RA
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y