Transcriptome Profiles of Populus euphratica upon Heat Shock stress.
Transcriptome Profiles of Populus euphratica upon Heat Shock stress.
复制标题
热休克胁迫下胡杨的转录组谱
DOI：
10.2174/138920291505141106101835
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发表时间：
2014-10
期刊：
影响因子：
2.6
通讯作者：
Xia X
中科院分区：
文献类型：
--
作者：
Chen J;Yin W;Xia X
高温胁迫严重影响植物的生长发育，导致植物营养生长下降和产量下降，已成为一个日益严重的全球性问题。 胡杨（Populus euphratica Oliv.该树种可耐受极宽的环境温度范围（-40 °C ~ 45 °C），是研究植物对非生物胁迫反应的树木模型。以往的研究主要集中在干旱和盐胁迫下的基因调控方面。然而，对热应激下基因在转录组水平的调控知之甚少。为了了解控制胡杨热胁迫的基因网络，利用Illumina Hiseq 2000对胡杨叶片组织中的每个样品进行了转录组测序，以产生10千兆碱基深度的序列。共获得119，573个单基因片段，平均长度为474 bp。约49，605（41.49%）个unigenes在两个文库中表现出显著差异的表达。在这些unigenes中，11，165个（9.34%）上调，38，440个（32.15%）下调。热休克蛋白被归类为分子伴侣，在上调组中显示出显著的百分比（1.13%）。热处理样品中多聚泛素等热响应基因表达量明显增加。GO富集分析表明，差异表达unigenes的GO项在激素介导的信号转导、生物过程调控和代谢过程调控方面显著富集。我们的数据揭示了胡杨对热激反应的全局转录组图。这些转录本可用于推测胡杨热应激分子机制的基因调控网络。
Heat stress, which strongly affects plant performance and often results in reduced vegetative growth and yields depression, has become an increasingly serious global problem. Populus euphratica Oliv. which has been considered as a tree model for the study of plant response to abiotic stresses, could be resistant to an extremely wide environmental temperature range (–40 °C to 45 °C). Previous study is mainly focused on its gene regulation upon drought and salt stress. However, little is known about gene regulation at the global transcriptome level upon heat stress. To understand the gene network controlling heat stress in P. euphratica, a transcriptome sequencing using Illumina Hiseq 2000 was performed to generate a 10 gigabases depth for each sample in the tissue of leaf. 119,573 unigeneswere generated with an average length of 474 bp. Approximately 49,605 (41.49%) unigenes exhibited significantly different expressions between two libraries. Among these unigenes, 11,165 (9.34%) were upregulated and 38,440 (32.15%) were down regulated. Heat shock proteins classified as molecular chaperones showed a significant percentage (1.13%) in the up regulated group. Heat responsive genes, such as polyubiquitins, were over expressed in heat treated sample. GO enrichment analysis revealed that the Go terms for differentially expressed unigenes were significantly enriched in hormone-mediated signal, biological process regulation and metabolic process regulation. Our data revealed a global transcriptome picture of P. euphratica in response to heat shock. The identified potential heat stress-related transcripts can be used to infer the gene regulation networks underlying the molecular mechanisms of heat response in P. euphratica.
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影响因子：
6.9
作者：
Krasensky J;Jonak C
通讯作者：
Jonak C
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
DOI：
10.1016/j.biocel.2012.03.009
发表时间：
2012-10-01
影响因子：
4
作者：
Morrow, Genevieve;Tanguay, Robert M.
通讯作者：
Tanguay, Robert M.
影响因子：
7
作者：
Li, Ruiqiang;Zhu, Hongmei;Wang, Jun
通讯作者：
Wang, Jun
影响因子：
2.5
作者：
Loser, K;Weltring, KM
通讯作者：
Weltring, KM