Glucosylated hydroxymethyluracil, DNA base J, prevents transcriptional readthrough in Leishmania.
Glucosylated hydroxymethyluracil, DNA base J, prevents transcriptional readthrough in Leishmania.
复制标题
DOI：
10.1016/j.cell.2012.07.030
复制
发表时间：
2012-08-31
期刊：
影响因子：
64.5
通讯作者：
Borst P
中科院分区：
文献类型：
--
作者：
van Luenen HG;Farris C;Jan S;Genest PA;Tripathi P;Velds A;Kerkhoven RM;Nieuwland M;Haydock A;Ramasamy G;Vainio S;Heidebrecht T;Perrakis A;Pagie L;van Steensel B;Myler PJ;Borst P
Some Ts in nuclear DNA of trypanosomes and Leishmania are hydroxylated and glucosylated to yield base J (β-D-glucosyl-hydroxymethyluracil). In Leishmania, about 99% of J is located in telomeric repeats. We show here that most of the remaining J is located at chromosome-internal RNA polymerase II termination sites. This internal J and telomeric J can be reduced by a knockout of J-binding protein 2 (JBP2), an enzyme involved in the first step of J biosynthesis. J levels are further reduced by growing Leishmania JBP2 knockout cells in BrdU-containing medium, resulting in cell death. The loss of internal J in JBP2 knockout cells is accompanied by massive readthrough at RNA polymerase II termination sites. The readthrough varies between transcription units but may extend over 100 kb. We conclude that J is required for proper transcription termination and infer that the absence of internal J kills Leishmania by massive readthrough of transcriptional stops.
登录
查看更多内容
影响因子：
48
作者：
Flusberg, Benjamin A.;Webster, Dale R.;Lee, Jessica H.;Travers, Kevin J.;Olivares, Eric C.;Clark, Tyson A.;Korlach, Jonas;Turner, Stephen W.
通讯作者：
Turner, Stephen W.
影响因子：
14.9
作者：
Heidebrecht T;Christodoulou E;Chalmers MJ;Jan S;Ter Riet B;Grover RK;Joosten RP;Littler D;van Luenen H;Griffin PR;Wentworth P Jr;Borst P;Perrakis A
通讯作者：
Perrakis A
影响因子：
56.9
作者：
Ivens, AC;Peacock, CS;Myler, PJ
通讯作者：
Myler, PJ
影响因子：
14.9
作者：
DELANGE, T;BERKVENS, TM;BORST, P
通讯作者：
BORST, P
影响因子：
14.9
作者：
Cliffe LJ;Kieft R;Southern T;Birkeland SR;Marshall M;Sweeney K;Sabatini R
通讯作者：
Sabatini R