Profiling of Antimicrobial Metabolites Synthesized by the Endophytic and Genetically Amenable Biocontrol Strain Bacillus velezensis DMW1.
Profiling of Antimicrobial Metabolites Synthesized by the Endophytic and Genetically Amenable Biocontrol Strain Bacillus velezensis DMW1.
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DOI：
10.1128/spectrum.00038-23
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发表时间：
2023-02-21
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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芽孢杆菌属是生物防治由各种植物病原体引起的植物病害的最重要的属之一。从马铃薯块茎内部组织中分离得到内生芽孢杆菌DMW1，具有较强的生防活性。根据其全基因组序列，DMW1 属于贝莱森芽孢杆菌 (Bacillus velezensis) 物种，与模型菌株贝莱森芽孢杆菌 FZB42 相似。在DMW1基因组中检测到12个次级代谢物生物合成基因簇（BGC），其中包括两个功能未知的BGC。该菌株被证明在遗传上是顺从的，并且通过遗传和化学相结合的方法鉴定了七种对抗植物病原体的次生代谢物。菌株DMW1确实显着改善了番茄和大豆幼苗的生长，并且能够控制植物幼苗中存在的大豆疫霉和青枯菌。由于这些特性，内生菌株 DMW1 似乎是与革兰氏阳性模型根际细菌 FZB42 一起进行比较研究的有希望的候选者，后者只能在根面定殖。重要性 植物病原体导致植物病害的广泛传播以及农作物产量的巨大损失。目前，用于控制植物病害的策略，包括开发抗性品种和化学防治，可能由于病原体的适应性进化而变得无效。因此，利用有益微生物来应对植物病害引起了人们的高度关注。在本研究中，发现了一种属于 B. velezensis 种的新菌株 DMW1，具有出色的生物防治特性。它显示出与温室条件下的 B. velezensis FZB42 相当的植物生长促进和疾病控制能力。根据基因组分析和生物活性代谢物分析，检测到负责促进植物生长的基因，并鉴定出具有不同拮抗活性的代谢物。我们的数据为DMW1作为生物农药的进一步开发和应用提供了基础，它与密切相关的模型菌株FZB42相似。
The genus Bacillus is one of the most important genera for the biological control of plant diseases that are caused by various phytopathogens. The endophytic Bacillus strain DMW1 was isolated from the inner tissues of potato tubers and exhibited strong biocontrol activity. Based on its whole-genome sequence, DMW1 belongs to the Bacillus velezensis species, and it is similar to the model strain B. velezensis FZB42. 12 secondary metabolite biosynthetic gene clusters (BGCs), including two unknown function BGCs, were detected in the DMW1 genome. The strain was shown to be genetically amenable, and seven secondary metabolites acting antagonistically against plant pathogens were identified by a combined genetic and chemical approach. Strain DMW1 did significantly improve the growth of tomato and soybean seedlings, and it was able to control the Phytophthora sojae and Ralstonia solanacearum that were present in the plant seedlings. Due to these properties, the endophytic strain DMW1 appears to be a promising candidate for comparative investigations performed together with the Gram-positive model rhizobacterium FZB42, which is only able to colonize the rhizoplane. IMPORTANCE Phytopathogens are responsible for the wide spread of plant diseases as well as for great losses of crop yields. At present, the strategies used to control plant disease, including the development of resistant cultivars and chemical control, may become ineffective due to the adaptive evolution of pathogens. Therefore, the use of beneficial microorganisms to deal with plant diseases attracts great attention. In the present study, a new strain DMW1, belonging to the species B. velezensis, was discovered with outstanding biocontrol properties. It showed plant growth promotion and disease control abilities that are comparable with those of B. velezensis FZB42 under greenhouse conditions. According to a genomic analysis and a bioactive metabolites analysis, genes that are responsible for promoting plant growth were detected, and metabolites with different antagonistic activities were identified. Our data provide a basis for DMW1 to be further developed and applied as a biopesticide, which is similar to the closely related model strain FZB42.
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影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
Yamada KD
影响因子：
5.5
作者：
Farzand, Ayaz;Moosa, Anam;Gao, Xuewen
通讯作者：
Gao, Xuewen
DOI：
10.18781/r.mex.fit.1706-5
发表时间：
2018-04-01
期刊：
Revista mexicana de fitopatología
影响因子：
--
作者：
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通讯作者：
Santos-Villalobos, Sergio de los
影响因子：
11.3
作者：
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通讯作者：
Crook DW
影响因子：
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作者：
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通讯作者：
Quecine, Maria Carolina