Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific.
Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific.
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DOI：
10.3389/fmicb.2013.00107
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发表时间：
2013
影响因子：
5.2
通讯作者：
Nakagawa S
中科院分区：
文献类型：
--
作者：
Mino S;Makita H;Toki T;Miyazaki J;Kato S;Watanabe H;Imachi H;Watsuji TO;Nunoura T;Kojima S;Sawabe T;Takai K;Nakagawa S
深海热液喷口是由微生物化学合成提供燃料的海底生物生产力高的区域。水生纲Persehonella属的成员是必需的化学合成细菌，在深海喷口的高温生境中似乎是碳、硫和氮循环的关键参与者。虽然这类细菌在世界各地的深海热液生态系统中分布广泛，但对其种内基因组多样性、分布格局和表型多样性等种群结构知之甚少。我们开发了多位点序列分析(MLSA)方案来研究它们的基因组特征。对来自冲绳海槽和南马里亚纳海槽(SNT)的36株嗜氢Persehonella菌株的5个看家基因和1个功能基因进行了序列分析。虽然这些菌株在16S rRNA基因序列上有98.7%的相似性，但MLSA显示了35种不同的序列类型(ST)，表明它们具有广泛的基因组多样性。从所有串联的基因序列推断的系统发育树揭示了根据地理来源的分离物的聚集性。此外，从全细胞基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF/MS)分析得出的表型聚集型模式可以与其MLSA聚集型模式相关联。这项研究是首次结合表型分析的MLSA，表明深海热液喷口细菌的异地物种形成。
Deep-sea hydrothermal vent fields are areas on the seafloor with high biological productivity fueled by microbial chemosynthesis. Members of the Aquificales genus Persephonella are obligately chemosynthetic bacteria, and appear to be key players in carbon, sulfur, and nitrogen cycles in high temperature habitats at deep-sea vents. Although this group of bacteria has cosmopolitan distribution in deep-sea hydrothermal ecosystem around the world, little is known about their population structure such as intraspecific genomic diversity, distribution pattern, and phenotypic diversity. We developed the multi-locus sequence analysis (MLSA) scheme for their genomic characterization. Sequence variation was determined in five housekeeping genes and one functional gene of 36 Persephonella hydrogeniphila strains originated from the Okinawa Trough and the South Mariana Trough (SNT). Although the strains share >98.7% similarities in 16S rRNA gene sequences, MLSA revealed 35 different sequence types (ST), indicating their extensive genomic diversity. A phylogenetic tree inferred from all concatenated gene sequences revealed the clustering of isolates according to the geographic origin. In addition, the phenotypic clustering pattern inferred from whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis can be correlated to their MLSA clustering pattern. This study represents the first MLSA combined with phenotypic analysis indicative of allopatric speciation of deep-sea hydrothermal vent bacteria.
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影响因子：
14.9
作者：
Ludwig, W;Strunk, O;Schleifer, KH
通讯作者：
Schleifer, KH
影响因子：
4.4
作者：
Kato, Shingo;Takano, Yoshinori;Yamagishi, Akihiko
通讯作者：
Yamagishi, Akihiko
影响因子：
11
作者：
Doroghazi, James R.;Buckley, Daniel H.
通讯作者：
Buckley, Daniel H.
影响因子：
2.1
作者：
Corre, E;Reysenbach, AL;Prieur, D
通讯作者：
Prieur, D
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.