Reduction in membranous expression of beta-catenin and increased cytoplasmic E-cadherin expression predict poor survival in gastric cancer.
Reduction in membranous expression of beta-catenin and increased cytoplasmic E-cadherin expression predict poor survival in gastric cancer.
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DOI：
10.1038/sj.bjc.6693437
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发表时间：
1999-12
影响因子：
8.8
通讯作者：
McCulloch, PG
中科院分区：
文献类型：
--
作者：
Ramesh, S;Nash, J;McCulloch, PG
β-catenin是E-cadherin-catenin细胞粘附复合体的一个组成部分，也起着单独的细胞内信号作用，与APC蛋白相互作用。结直肠肿瘤中β-连环蛋白的细胞内积聚很常见。β-catenin异常与胃癌的低生存率相关，但先前的研究并未区分膜相关β-catenin和细胞内β-catenin。在这项研究中，我们旨在确定哪种类型的E-cadherin， β-catenin和α-catenin的表达异常与胃癌的临床病理特征和生存相关。采用微波揭膜和亲和生物素技术对40例胃癌石蜡切片进行E-cadherin、α-和β-catenins的免疫过氧化物酶染色。临床资料来源于病例记录和癌症登记记录。β-catenin在10/12（83%）弥漫性肠肿瘤和8/28（29%）肠肿瘤中膜表达减少（P = 0.0014），与分化差（P = 0.0015）和生存期短（P = 0.032）相关，但与年龄、性别、肿瘤大小或淋巴结状态无关。β-catenin核表达少见；在13/40（33%）的病例中观察到细胞质表达，但与组织学、肿瘤分级或生存无关。E-cadherin膜表达降低与淋巴结转移相关（P = 0.02）。E-cadherin和α-catenin的表达均与生存率无关。胃癌中β-catenin膜表达降低预示预后不良，而细胞内异位表达相对少见。β-catenin表达与结肠癌中发现的明显差异值得进一步研究。©1999癌症研究运动
β-catenin, a component of the E-cadherin–catenin cell adhesion complex, also plays a separate intracellular signalling role, interacting with APC protein. Intracellular accumulation of β-catenin is common in colorectal neoplasia. β-catenin abnormalities are associated with poor survival in gastric cancer, but previous studies do not differentiate between membrane-associated and intracellular β-catenin. In this study we aimed to determine which type of expression abnormalities for E-cadherin, β-catenin and α-catenin correlate with clinico-pathological features and survival in gastric cancer. Immunoperoxidase staining of paraffin-embedded sections from 40 gastric cancers was performed for E-cadherin, α- and β-catenins using microwave unmasking and an avidin–biotin technique. Clinical data were obtained from case records and cancer registry records. Reduced membranous expression of β-catenin occurred in 10/12 (83%) diffuse and 8/28 (29%) intestinal tumours (P = 0.0014), and was associated with poor differentiation (P = 0.0015) and short survival (P = 0.032), but not with age, sex, tumour size or nodal status. Nuclear expression of β-catenin was uncommon; cytoplasmic expression was observed in 13/40 cases (33%) but did not correlate with histology, tumour grade or survival. Reduced E-cadherin membrane expression was associated with lymph node metastasis (P = 0.02). Neither E-cadherin or α-catenin expression correlated with survival. Reduced membranous expression of β-catenin predicts poor prognosis in gastric cancer, whilst ectopic intracellular expression is relatively rare. The apparent differences in β-catenin expression from those found in colon cancer merit further study. © 1999 Cancer Research Campaign
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影响因子：
7.8
作者：
Hinck, L;Nelson, W J;Papkoff, J
通讯作者：
Papkoff, J
影响因子：
8.8
作者：
Matsuura, K;Kawanishi, J;Fujii, S;Imamura, M;Hirano, S;Takeichi, M;Niitsu, Y
通讯作者：
Niitsu, Y
DOI：
10.1073/pnas.92.7.3046
发表时间：
1995-03-28
影响因子：
11.1
作者：
MUNEMITSU, S;ALBERT, I;POLAKIS, P
通讯作者：
POLAKIS, P
影响因子：
3.5
作者：
NAKATSURU, S;YANAGISAWA, A;HORII, A
通讯作者：
HORII, A
DOI：
10.1111/apm.1965.64.1.31
发表时间：
1965-01-01
期刊：
ACTA PATHOLOGICA ET MICROBIOLOGICA SCANDINAVICA
影响因子：
--
作者：
LAUREN, P
通讯作者：
LAUREN, P