Use of Zebrafish Larvae as a Multi-Endpoint Platform to Characterize the Toxicity Profile of Silica Nanoparticles.
Use of Zebrafish Larvae as a Multi-Endpoint Platform to Characterize the Toxicity Profile of Silica Nanoparticles.
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DOI：
10.1038/srep37145
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发表时间：
2016-11-22
影响因子：
4.6
通讯作者：
de Witte PA
中科院分区：
文献类型：
--
作者：
Pham DH;De Roo B;Nguyen XB;Vervaele M;Kecskés A;Ny A;Copmans D;Vriens H;Locquet JP;Hoet P;de Witte PA
纳米材料正在广泛生产并应用于社会。因此，人类和环境的暴露是不可避免的，因此人们越来越关注纳米毒性。虽然二氧化硅纳米粒子 (NP) 是消费品和生物医学产品中发现的最常见的五种纳米材料之一，但其毒性特征却很少被表征。在这项研究中，我们使用体内斑马鱼平台研究了直径为 20、50 和 80 nm 的二氧化硅纳米颗粒的毒性，该平台分析了与发育、心脏、肝脏和神经毒性相关的多个终点。结果表明，除了加速孵化时间和改变斑马鱼胚胎/幼虫的行为外，二氧化硅纳米颗粒不会引起任何发育缺陷，也不会引起任何心脏和肝脏毒性。胚胎光运动和幼虫运动反应的行为改变是一致的，并且取决于二氧化硅纳米粒子的浓度和大小。由于胚胎和幼虫表现出正常的触摸反应，并且早期孵化并不影响幼虫的运动反应，因此观察到的行为变化很可能是神经活动改变的结果。总的来说，我们的结果表明二氧化硅纳米颗粒不会引起任何发育、心脏或肝脏毒性，但它们对神经行为系统构成潜在风险。
Nanomaterials are being extensively produced and applied in society. Human and environmental exposures are, therefore, inevitable and so increased attention is being given to nanotoxicity. While silica nanoparticles (NP) are one of the top five nanomaterials found in consumer and biomedical products, their toxicity profile is poorly characterized. In this study, we investigated the toxicity of silica nanoparticles with diameters 20, 50 and 80 nm using an in vivo zebrafish platform that analyzes multiple endpoints related to developmental, cardio-, hepato-, and neurotoxicity. Results show that except for an acceleration in hatching time and alterations in the behavior of zebrafish embryos/larvae, silica NPs did not elicit any developmental defects, nor any cardio- and hepatotoxicity. The behavioral alterations were consistent for both embryonic photomotor and larval locomotor response and were dependent on the concentration and the size of silica NPs. As embryos and larvae exhibited a normal touch response and early hatching did not affect larval locomotor response, the behavior changes observed are most likely the consequence of modified neuroactivity. Overall, our results suggest that silica NPs do not cause any developmental, cardio- or hepatotoxicity, but they pose a potential risk for the neurobehavioral system.
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影响因子：
4.5
作者：
Clemente, Z.;Castro, V. L. S. S.;Fraceto, L. F.
通讯作者：
Fraceto, L. F.
DOI：
10.1523/jneurosci.3689-12.2013
发表时间：
2013-02-27
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Kokel D;Dunn TW;Ahrens MB;Alshut R;Cheung CY;Saint-Amant L;Bruni G;Mateus R;van Ham TJ;Shiraki T;Fukada Y;Kojima D;Yeh JR;Mikut R;von Lintig J;Engert F;Peterson RT
通讯作者：
Peterson RT
影响因子：
2.7
作者：
LEUNG, TS;BULKLEY, RV
通讯作者：
BULKLEY, RV
影响因子：
3.5
作者：
Her, GM;Chiang, CC;Wu, JL
通讯作者：
Wu, JL
影响因子：
17.1
作者：
Lee, Kerry J.;Nallathamby, Prakash D.;Xu, Xiao-Hong Nancy
通讯作者：
Xu, Xiao-Hong Nancy