Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes.
Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes.
复制标题
NACL胁迫的拟南芥根的全面转录分析揭示了新的反应基因类别。
DOI：
10.1186/1471-2229-6-25
复制
发表时间：
2006-10-12
影响因子：
5.3
通讯作者：
Deyholos, Michael K.
中科院分区：
文献类型：
--
作者：
Jiang, Yuanqing;Deyholos, Michael K.
由于根系在农业中的重要地位，以及其相对结构和生物化学上的简单性，根系是NaCl响应的基因组和后基因组研究的一个有吸引力的系统。我们已经为拟南芥根系的研究建立了优秀的基因组资源，然而，为了进一步了解植物对非生物胁迫的反应，并促进未来基于系统的潜在调控网络分析，需要对NaCl暴露后的根系转录组进行全面的微阵列分析。研究人员使用70-mer寡核苷酸探针微阵列，代表23,686个拟南芥基因，以鉴定在150 mM NaCl水培暴露6小时、24小时或48小时后相对丰度发生变化的根转录物。富集分析确定了结构上或功能上相关的基因群，其成员在统计上过度代表了上调或下调的转录本。我们的研究结果与通常观察到的应激反应主题一致，并强调了未被重视的基因家族的潜在重要作用，包括：几组转运蛋白（例如MATE， leopt1样）；信号分子（如PERK激酶、mlo样受体）、碳水化合物活性酶（如XTH18）、转录因子（如ZIM、WRKY、NAC的成员）和其他蛋白质（如4cl样、comt样、LOB-Class 1）。我们通过qRT-PCR验证了nacl诱导的转录因子和其他基因的表达。对nacl处理的拟南芥根系的微阵列分析显示，至少20%的基因组转录物丰度发生了动态变化，包括数百个转录因子、激酶/磷酸酶、激素相关基因和稳态效应物，所有这些都突出了这种胁迫反应的复杂性。我们对这些转录反应的鉴定，以及对胁迫具有相似或不同转录反应的进化相关基因群，将有助于绘制调控网络，并扩展我们提高植物耐盐性的能力。
Roots are an attractive system for genomic and post-genomic studies of NaCl responses, due to their primary importance to agriculture, and because of their relative structural and biochemical simplicity. Excellent genomic resources have been established for the study of Arabidopsis roots, however, a comprehensive microarray analysis of the root transcriptome following NaCl exposure is required to further understand plant responses to abiotic stress and facilitate future, systems-based analyses of the underlying regulatory networks. We used microarrays of 70-mer oligonucleotide probes representing 23,686 Arabidopsis genes to identify root transcripts that changed in relative abundance following 6 h, 24 h, or 48 h of hydroponic exposure to 150 mM NaCl. Enrichment analysis identified groups of structurally or functionally related genes whose members were statistically over-represented among up- or down-regulated transcripts. Our results are consistent with generally observed stress response themes, and highlight potentially important roles for underappreciated gene families, including: several groups of transporters (e.g. MATE, LeOPT1-like); signalling molecules (e.g. PERK kinases, MLO-like receptors), carbohydrate active enzymes (e.g. XTH18), transcription factors (e.g. members of ZIM, WRKY, NAC), and other proteins (e.g. 4CL-like, COMT-like, LOB-Class 1). We verified the NaCl-inducible expression of selected transcription factors and other genes by qRT-PCR. Micorarray profiling of NaCl-treated Arabidopsis roots revealed dynamic changes in transcript abundance for at least 20% of the genome, including hundreds of transcription factors, kinases/phosphatases, hormone-related genes, and effectors of homeostasis, all of which highlight the complexity of this stress response. Our identification of these transcriptional responses, and groups of evolutionarily related genes with either similar or divergent transcriptional responses to stress, will facilitate mapping of regulatory networks and extend our ability to improve salt tolerance in plants.
登录
查看更多内容
影响因子：
--
作者：
Gould KS
通讯作者：
Gould KS
DOI：
10.1073/pnas.260502697
发表时间：
2000-12-19
影响因子：
11.1
作者：
Barlier, I;Kowalczyk, M;Bellini, C
通讯作者：
Bellini, C
影响因子：
4.8
作者：
Chen, KM;Gong, HJ;Zhang, CL
通讯作者：
Zhang, CL
影响因子：
7.2
作者：
Gilmour, SJ;Zarka, DG;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
11.6
作者：
Chen, WQ;Provart, NJ;Zhu, T
通讯作者：
Zhu, T