Functional Val66Met polymorphism of Brain-derived neurotrophic factor in type 2 diabetes with depression in Han Chinese subjects.
Functional Val66Met polymorphism of Brain-derived neurotrophic factor in type 2 diabetes with depression in Han Chinese subjects.
复制标题
中国汉族2型糖尿病伴抑郁患者脑源性神经营养因子功能性Val66Met多态性
DOI：
10.1186/1744-9081-9-34
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发表时间：
2013-08-22
期刊：
影响因子：
--
通讯作者：
Chen LM
中科院分区：
文献类型：
--
作者：
Zhou JX;Li HC;Bai XJ;Chang BC;Li CJ;Sun P;Chen LM
背景脑源性神经营养因子（BDNF）参与了抑郁症的发病机制。2型糖尿病（T2 DM）患者的重度抑郁症患病率较高，BDNF水平较低。因此，本研究旨在探讨BDNF Val 66 Met基因多态性与抑郁症的相关性，以及抑郁症是否影响中国汉族2型糖尿病患者血清BDNF水平。方法选取296例2型糖尿病患者和70例健康志愿者（健康对照组）作为研究对象。根据流行病学研究中心抑郁量表（CES-D）和患者健康问卷（PHQ-9）评估是否存在抑郁，将T2 DM患者分为抑郁性糖尿病组（DDM组，n = 64）和非抑郁性糖尿病组（NDDM组，n = 232）。采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）检测Val 66 Met基因多态性。结果296例T2 DM患者中有21.6%（64/296）存在抑郁症状。BDNF Val 66 Met基因型在三组间的分布差异有统计学意义（χ2= 7.39，p < 0.05）。DDM组Met等位基因频率（53.9%）高于HC组（39.3%）和NDDM组（38.8%）。与具有瓦尔/Met（79.04 ± 5.19 pg/ml）和瓦尔/瓦尔（83.83 ± 3.97 pg/ml）的受试者相比，具有Met/Met的受试者具有最低的血清BDNF水平（76.59 ± 5.12 pg/ml，F = 7.39，p < 0.05）。2型糖尿病组中，DDM组血清BDNF水平明显低于NDDM组（76.67 ± 5.35 vs 79.84 ± 3.97 pg/ml，p < 0.05）。在2型糖尿病患者中，BDNF血清水平与基因型（r =-0.346，p < 0.01）、抑郁评分（r =-0.486，p < 0.01）和HbA 1c（r =-0.168，p < 0.05）显著相关。校正性别、HbA 1c、BMI和并发症数量后，BDNF瓦尔/Met基因型分布（OR = 2.105，p < 0.05）和血清BDNF水平降低（OR = 0.835，p < 0.01），但不影响正常工作。结论BDNFVal 66 Met基因多态性可能通过降低T2 DM患者血清BDNF水平而参与T2 DM患者抑郁的发生。汉族受试者。
BackgroundBrain-derived neurotrophic factor (BDNF) has been implicated in the pathogenesis of major depression. Individuals with type 2 diabetes (T2DM) have a high prevalence of major depression and low levels of BDNF. We therefore explored whether the BDNF Val66Met polymorphism is associated with co-morbid depression and whether depression affects the serum levels of BDNF in a Han Chinese subjects with T2DM.MethodsA Total of 296 T2DM patients and 70 healthy volunteers (Health control, HC group) were recruited in this study. T2DM patients were divided into two subgroups: depressive diabetes group (DDM group, n = 64) and non-depressive diabetes group (NDDM group, n = 232), according to the presence or the absence of depression assessed by Center for Epidemiologic Studies Depression Scale (CES-D) and Patient Health Questionnaire-9 (PHQ-9). Val66Met polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP). Serum BDNF levels were measured by ELISA kit.ResultsIn this study, 21.6% (64/296) patients with T2DM had depression. The BDNF Val66Met genotype distributions were statistically different among the three groups (χ2= 7.39, p < 0.05). DDM group carried the highest frequencies of Met allele (53.9%) compared to HC group (39.3%) and NDDM group (38.8%). Subjects with Met/Met had lowest serum BDNF levels (76.59 ± 5.12 pg/ml, F = 7.39, p < 0.05) compared to subjects with Val/Met (79.04 ± 5.19 pg/ml) and Val/Val (83.83 ± 3.97 pg/ml). Within T2DM group, it was also observed that the serum BDNF levels in DDM group were significantly lower than those in NDDM group (76.67 ± 5.35 vs. 79.84 ± 3.97 pg/ml, p < 0.05). In type 2 diabetes subjects, BDNF serum levels were significant correlations with genotypes (r = −0.346, p < 0.01), depression scores (r = −0.486, p < 0.01) and HbA1c (r = −0.168, p < 0.05). After adjustment for gender, HbA1c, BMI and numbers of complications, BDNF Val/Met genotype distributions (OR = 2.105, p < 0.05) and decreased serum BDNF levels (OR = 0.835, p < 0.01) were independently associated with depression in T2DM.ConclusionsThe BDNF Val66Met polymorphism might be implicated in the pathogenesis of depression in T2DM by decreasing serum BDNF levels in Han Chinese Subjects.
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