Deep Penetration Microscopic Imaging with Non-Diffracting Airy Beams.
Deep Penetration Microscopic Imaging with Non-Diffracting Airy Beams.
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DOI：
10.3390/membranes11060391
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发表时间：
2021-05-26
期刊：
影响因子：
4.2
通讯作者：
Qu J
中科院分区：
文献类型：
--
作者：
Guo Y;Huang Y;Li J;Wang L;Yang Z;Liu J;Peng X;Yan W;Qu J
我们报道了一种无衍射艾利光束的深穿透显微成像方法。体成像在自由空间的直接映射表明，在保持较窄的横向宽度的情况下，艾里光束的轴向成像范围约为传统高斯光束的4倍。艾里束照明显微成像具有无衍射性，可以通过混浊介质获取图像结构，并在单帧图像中捕捉到一幅体图像。我们通过633 nm和780 nm激光的强散射环境演示了艾里显微成像的穿透能力。利用HeLa细胞和分离的小鼠肾组织对体积成像方法的性能进行了评价。厚样品在高斯模式下被逐层扫描，而在艾里模式下，三维(3D)结构信息被投影到二维(2D)图像上，这大大提高了体积成像的速度。为了展示艾里显微镜的特性，我们用双光子对分离的小鼠肾组织进行了动态体积成像。
We report a deep penetration microscopic imaging method with a non-diffracting Airy beam. The direct mapping of volume imaging in free space shows that the axial imaging range of the Airy beam is approximately 4 times that of the traditional Gaussian beam along the axial direction while maintaining a narrow lateral width. Benefiting from its non-diffracting property, the microscopic imaging with Airy beam illumination can acquire image structures through turbid medium and capture a volumetric image in a single frame. We demonstrate the penetration ability of the Airy microscopic imaging through a strongly scattering environment with 633 nm and 780 nm lasers. The performances of the volumetric imaging method were evaluated using HeLa cells and isolated mouse kidney tissue. The thick sample was scanned layer by layer in the Gaussian mode, however, in the Airy mode, the three-dimensional (3D) structure information was projected onto a two-dimensional (2D) image, which vastly increased the volume imaging speed. To show the characteristics of the Airy microscope, we performed dynamic volumetric imaging on the isolated mouse kidney tissue with two-photon.
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