Separation and identification of neutral oligosaccharides with prebiotic activities from apple pectin
Separation and identification of neutral oligosaccharides with prebiotic activities from apple pectin
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苹果果胶中具有益生元活性的中性低聚糖的分离与鉴定
DOI：
10.1016/j.foodhyd.2021.107062
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发表时间：
2021-12
影响因子：
10.7
通讯作者：
Ning He
中科院分区：
文献类型：
--
作者：
Huipeng Liu;Xiaoyu Wei;Shuyu Zu;Xihuang Lin;Jinchuang Zhang;Aimin Shi;Qiang Wang;Ning He
基于其聚合度 (DP) 和结构复杂性，植物源性中性低聚糖和果胶低聚糖 (POS) 被建议作为益生元。在本研究中，使用优化的果胶酶浓度和降解时间，从商业苹果果胶中生产出不含半乳糖醛酸的中性寡糖。使用 DEAE-Sepharose CL-6B 柱、石墨碳柱和 Xbridge C18 柱对粗寡糖溶液进行膜分离精制，得到 DP 3-13 的中性寡糖。寡糖的单糖组成主要包括葡萄糖、阿拉伯糖、半乳糖和木糖，不含半乳糖醛酸。使用具有电喷雾电离源的质谱法、通过HILIC柱上的UPLC和1 H NMR来分析寡糖的结构。正离子模式的MS/MS谱和1H NMR显示主要寡糖结构对应于阿拉伯半乳聚糖与Galβ1→4Galβ1→4Galβ1→4Galβ1→α3Araf的连接。所选乳酸菌的生长。添加DP=3-13的精制中性低聚糖与市售菊粉相比，菌株得到改良，大肠杆菌生长基本不生长。因此，中性寡糖（DP=3-13）可以被认为是有效的益生元，在功能性食品中具有潜在的应用前景。
Plant-derived neutral oligosaccharides and pectic-oligosaccharides (POS) have been suggested as prebiotics based on their degree of polymerization (DP) and structural complexity. In the present study, neutral oligosaccharides without galacturonic acid were produced from commercial apple pectin using an optimized concentration of pectinase and degradation time. The crude oligosaccharide solution was refined by membrane separation using a DEAE-Sepharose CL-6B column, a graphite carbon column and an Xbridge C18 column, which resulted in the neutral oligosaccharides with DP 3–13. The monosaccharide composition of oligosaccharides primarily included glucose, arabinose, galactose and xylose without galacturonic acid. The structure of the oligosaccharides was analyzed using mass spectrometry with an electrospray ionization source by UPLC on an HILIC column and1H NMR. The MS/MS spectra in positive ion mode and1H NMR showed a main oligosaccharide structure corresponding to the connection of arabinogalactan with Galβ1→4Galβ1→4Galβ1→4Galβ1→α3Araf. The growth of the selected Lactobacillus. strains was improved by adding refined neutral oligosaccharides with DP = 3–13 compared with commercial inulin, and growth ofEscherichia colihad not basically grown. Therefore, neutral oligosaccharides (DP = 3–13) can be considered effective prebiotics for potential application in functional food.
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影响因子：
10.7
作者：
Yeung, Yuen Kwan;Kang, Yu-Ra;Chang, Yoon Hyuk
通讯作者：
Chang, Yoon Hyuk
影响因子：
4
作者：
Al-Tamimi, MAHM;Palframan, RJ;Rastall, RA
通讯作者：
Rastall, RA
影响因子：
11.2
作者：
I. Sims;A. M. Gane;D. Dunstan;G. C. Allan;D. V. Boger;L. Melton;A. Bacic
通讯作者：
I. Sims;A. M. Gane;D. Dunstan;G. C. Allan;D. V. Boger;L. Melton;A. Bacic
影响因子：
11.2
作者：
Kost'alova, Zuzana;Hromadkova, Zdenka;Ebringerova, Anna
通讯作者：
Ebringerova, Anna
影响因子：
6.1
作者：
Holck, Jesper;Lorentzen, Andrea;Meyer, Anne S.
通讯作者：
Meyer, Anne S.