A candidate gene based approach validates Md-PG1 as the main responsible for a QTL impacting fruit texture in apple (Malus x domestica Borkh).
A candidate gene based approach validates Md-PG1 as the main responsible for a QTL impacting fruit texture in apple (Malus x domestica Borkh).
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DOI：
10.1186/1471-2229-13-37
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发表时间：
2013-03-04
影响因子：
5.3
通讯作者：
Costa F
中科院分区：
文献类型：
--
作者：
Longhi S;Hamblin MT;Trainotti L;Peace CP;Velasco R;Costa F
苹果因其优良的品质和耐贮性而被广泛种植。在几个品质因子中，质地是最重要和最受赞赏的，并且在苹果品种全景中，皮层质地显示出广泛的变异性。解剖学上，这些变化取决于降解事件发生在果实初生细胞壁和中层。这一生理过程是由一个酶网络调控的，该酶网络通常由大的基因家族编码，其中多聚半乳糖醛酸酶致力于果胶的解聚。在苹果中，Md-PG 1，属于多聚半乳糖醛酸酶基因家族的关键基因，被定位在第10号染色体上，并共定位在一个与几个果实质地亚表型相关的主要热点QTL的统计区间内。本研究在77个苹果品种中验证了Md-PG 1位点的QTL，发现了与果实质地性状相关的新的功能等位基因。采用基因全长重测序法对38个SNPs进行基因分型，并利用2个SSR标记对基因后桥进行特异性检测。在这个SNP组中，11个被用来定义统计学上与几个纹理成分相关的三个显著的单倍型。通过对质地表现相反的两个苹果品种“金冠”和“史密斯奶奶”的皮层结构电镜扫描，进一步证实了Md-PG 1对果实细胞壁解体的影响。研究结果为苹果果实质地的遗传解剖提供了新的思路.这套新的单倍型和微卫星等位基因，可以代表一个有价值的工具箱，更有效的亲本选择，以及鉴定新的苹果加入区分的上级水果品质特征。
Apple is a widely cultivated fruit crop for its quality properties and extended storability. Among the several quality factors, texture is the most important and appreciated, and within the apple variety panorama the cortex texture shows a broad range of variability. Anatomically these variations depend on degradation events occurring in both fruit primary cell wall and middle lamella. This physiological process is regulated by an enzymatic network generally encoded by large gene families, among which polygalacturonase is devoted to the depolymerization of pectin. In apple, Md-PG1, a key gene belonging to the polygalacturonase gene family, was mapped on chromosome 10 and co-localized within the statistical interval of a major hot spot QTL associated to several fruit texture sub-phenotypes. In this work, a QTL corresponding to the position of Md-PG1 was validated and new functional alleles associated to the fruit texture properties in 77 apple cultivars were discovered. 38 SNPs genotyped by gene full length resequencing and 2 SSR markers ad hoc targeted in the gene metacontig were employed. Out of this SNP set, eleven were used to define three significant haplotypes statistically associated to several texture components. The impact of Md-PG1 in the fruit cell wall disassembly was further confirmed by the cortex structure electron microscope scanning in two apple varieties characterized by opposite texture performance, such as ‘Golden Delicious’ and ‘Granny Smith’. The results here presented step forward into the genetic dissection of fruit texture in apple. This new set of haplotypes, and microsatellite alleles, can represent a valuable toolbox for a more efficient parental selection as well as the identification of new apple accessions distinguished by superior fruit quality features.
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影响因子：
3.3
作者：
Breseghello, F;Sorrells, ME
通讯作者：
Sorrells, ME
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
5.3
作者：
Costa F;Alba R;Schouten H;Soglio V;Gianfranceschi L;Serra S;Musacchi S;Sansavini S;Costa G;Fei Z;Giovannoni J
通讯作者：
Giovannoni J
影响因子：
4.4
作者：
Chagne, David;Gasic, Ksenija;Korban, Schuyler S.
通讯作者：
Korban, Schuyler S.
影响因子：
1.9
作者：
Costa, F;Stella, S;Sansavini, S
通讯作者：
Sansavini, S