TD/GC-MS analysis of volatile markers emitted from mono- and co-cultures of Enterobacter cloacae and Pseudomonas aeruginosa in artificial sputum.
TD/GC-MS analysis of volatile markers emitted from mono- and co-cultures of Enterobacter cloacae and Pseudomonas aeruginosa in artificial sputum.
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DOI：
10.1007/s11306-018-1357-5
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
BreathDx consortium
中科院分区：
文献类型：
--
作者：
Lawal O;Knobel H;Weda H;Nijsen TME;Goodacre R;Fowler SJ;BreathDx consortium
呼吸机相关性肺炎（VAP）等感染可由一种或多种病原体引起。目前鉴定这些致病微生物的方法通常需要侵入性取样，并且由于需要长时间的培养富集以及选择性和差异电镀步骤，可能非常耗时。这导致诊断延误，对这些危重病人可能造成潜在的危及生命的后果。因此，需要一种无创、及时的诊断方法。提出了呼气中微生物挥发性有机化合物（VOCs）的检测作为鉴定这些病原体的替代方法，可以区分单微生物感染和多微生物感染。研究区分细菌单一和共同培养的挥发性代谢物。将阴沟肠杆菌和铜绿假单胞菌分别和一起在人工痰液中培养24 h，采用热解吸气相色谱-质谱法分析其顶空是否存在潜在的区别性VOCs。在推定鉴定的70种挥发性有机化合物中，发现23种在细菌培养过程中显著增加（即可能在代谢过程中释放），13种在代谢过程中减少（即可能在代谢过程中消耗）。其他挥发性有机化合物没有发生转化（与介质中观察到的浓度相似）。细菌特异性的挥发性有机化合物包括2-甲基-1-丙醇、2-苯乙醇和3-甲基-1-丁醇，在阴沟芽孢杆菌无菌培养物的顶空中观察到，在铜绿假单胞菌培养物的顶空中观察到2-乙基己酸甲酯，这是本研究的新发现。之前报道的VOCs 1-十一烯和吡咯也被检测到。据报道，代谢物2-甲基乙酸丁酯和2-甲基丁酸甲酯具有抗菌活性，但仅在共培养中升高。观察到的VOCs能够区分无性和共培养。在呼出样本中验证这些标记物可用于及时鉴定病原体和诊断感染类型。本文的在线版本（10.1007/s11306-018-1357-5）包含补充内容，授权用户可使用。
Infections such as ventilator-associated pneumonia (VAP) can be caused by one or more pathogens. Current methods for identifying these pathogenic microbes often require invasive sampling, and can be time consuming, due to the requirement for prolonged cultural enrichment along with selective and differential plating steps. This results in delays in diagnosis which in such critically ill patients can have potentially life-threatening consequences. Therefore, a non-invasive and timely diagnostic method is required. Detection of microbial volatile organic compounds (VOCs) in exhaled breath is proposed as an alternative method for identifying these pathogens and may distinguish between mono- and poly-microbial infections. To investigate volatile metabolites that discriminate between bacterial mono- and co-cultures. VAP-associated pathogens Enterobacter cloacae and Pseudomonas aeruginosa were cultured individually and together in artificial sputum medium for 24 h and their headspace was analysed for potential discriminatory VOCs by thermal desorption gas chromatography–mass spectrometry. Of the 70 VOCs putatively identified, 23 were found to significantly increase during bacterial culture (i.e. likely to be released during metabolism) and 13 decreased (i.e. likely consumed during metabolism). The other VOCs showed no transformation (similar concentrations observed as in the medium). Bacteria-specific VOCs including 2-methyl-1-propanol, 2-phenylethanol, and 3-methyl-1-butanol were observed in the headspace of axenic cultures of E. cloacae, and methyl 2-ethylhexanoate in the headspace of P. aeruginosa cultures which is novel to this investigation. Previously reported VOCs 1-undecene and pyrrole were also detected. The metabolites 2-methylbutyl acetate and methyl 2-methylbutyrate, which are reported to exhibit antimicrobial activity, were elevated in co-culture only. The observed VOCs were able to differentiate axenic and co-cultures. Validation of these markers in exhaled breath specimens could prove useful for timely pathogen identification and infection type diagnosis. The online version of this article (10.1007/s11306-018-1357-5) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Culotti A;Packman AI
通讯作者：
Packman AI
DOI：
10.1186/cc13775
发表时间：
2014-03-18
期刊：
Critical care (London, England)
影响因子：
--
作者：
Kalanuria AA;Ziai W;Mirski M
通讯作者：
Mirski M
影响因子：
1.5
作者：
Leininger, DJ;Roberson, JR;Elvinger, F
通讯作者：
Elvinger, F
影响因子：
30.3
作者：
Littman DR;Pamer EG
通讯作者：
Pamer EG
影响因子：
6.4
作者：
Martín R;Miquel S;Ulmer J;Kechaou N;Langella P;Bermúdez-Humarán LG
通讯作者：
Bermúdez-Humarán LG