Switching of α-Catenin From Epithelial to Neuronal Type During Lens Epithelial Cell Differentiation.
Switching of α-Catenin From Epithelial to Neuronal Type During Lens Epithelial Cell Differentiation.
复制标题
晶状体上皮细胞分化过程中 α-连环蛋白从上皮型向神经元型的转变。
DOI：
10.1167/iovs.17-21539
复制
发表时间：
2017-07-01
影响因子：
4.4
通讯作者：
Rao PV
中科院分区：
文献类型：
--
作者：
Maddala R;Rao PV
眼晶状体纤维细胞的伸长、分化和压缩与细胞粘附相互作用和细胞骨架的广泛重组有关；然而，我们对这些事件至关重要的蛋白质的了解仍在不断发展。本研究描述了神经元特异性 α-连环蛋白 (αN-连环蛋白) 的分布模式及其与 N-钙粘蛋白相关粘附连接 (AJ) 的相互作用及其在小鼠晶状体纤维中的稳定性。通过 RT-PCR、免疫印迹和免疫荧光分析确定 αN-连环蛋白在发育中的小鼠和成人晶状体中的表达和分布。使用免疫沉淀、质谱和共聚焦成像对 αN-连环蛋白和 N-钙粘蛋白相互作用蛋白进行表征和共定位分析。使用 Perixin-null 小鼠评估了 Periaxin 缺乏对晶状体纤维细胞 AJ 稳定性的影响。 αN-连环蛋白在小鼠和人类晶状体中均呈现离散分布，在纤维细胞分化和成熟过程中经历强烈的上调。相反，上皮特异性 α-连环蛋白（αE-连环蛋白）主要分布于晶状体上皮。 αN-连环蛋白和 N-钙粘蛋白与 β-连环蛋白、肌动蛋白、血影蛋白、粘蛋白、在 velo-cardio-facial 综合征同源物中删除的犰狳重复蛋白、periaxin 和晶状体纤维中的锚蛋白-B 相互共免疫沉淀和共定位。来自 periaxin-null 小鼠晶状体的纤维细胞显示基于 N-钙粘蛋白/αN-连环蛋白的 AJ 受到破坏。这些结果表明，在晶状体上皮细胞分化过程中，α-连环蛋白表达从 αE-连环蛋白向 αN-连环蛋白亚型的离散转变可能通过调节基于 N-钙粘蛋白的 AJ 的组装和稳定性，在纤维细胞结构中发挥关键作用。这项研究还提供了证据，证明纤维细胞特异性细胞骨架相互作用的 periaxin 在晶状体纤维中基于 N-钙粘蛋白/αN-连环蛋白的 AJ 的稳定性中的重要性。
Ocular lens fiber cell elongation, differentiation, and compaction are associated with extensive reorganization of cell adhesive interactions and cytoskeleton; however, our knowledge of proteins critical to these events is still evolving. This study characterizes the distribution pattern of neuronal-specific α-catenin (αN-catenin) and its interaction with the N-cadherin–associated adherens junctions (AJs) and their stability in the mouse lens fibers. Expression and distribution of αN-catenin in developing mouse and adult human lenses was determined by RT-PCR, immunoblot, and immunofluorescence analyses. Characterization of αN-catenin and N-cadherin interacting proteins and colocalization analyses were performed using immunoprecipitation, mass spectrometry, and confocal imaging. Effects of periaxin deficiency on the stability of lens fiber cell AJs were evaluated using perixin-null mice. αN-catenin exhibits discrete distribution to lens fibers in both mouse and human lenses, undergoing a robust up-regulation during fiber cell differentiation and maturation. Epithelial-specific α-catenin (αE-catenin), in contrast, distributes primarily to the lens epithelium. αN-catenin and N-cadherin reciprocally coimmunoprecipitate and colocalize along with β-catenin, actin, spectrin, vinculin, Armadillo repeat protein deleted in velo-cardio-facial syndrome homolog, periaxin, and ankyrin-B in lens fibers. Fiber cells from periaxin-null mouse lenses revealed disrupted N-cadherin/αN-catenin–based AJs. These results suggest that the discrete shift in α-catenin expression from αE-catenin to αN-catenin subtype that occurs during lens epithelial cell differentiation may play a key role in fiber cell cytoarchitecture by regulating the assembly and stability of N-cadherin–based AJs. This study also provides evidence for the importance of the fiber cell–specific cytoskeletal interacting periaxin, in the stability of N-cadherin/αN-catenin–based AJs in lens fibers.
登录
查看更多内容
影响因子：
16.2
作者：
GILLESPIE, CS;SHERMAN, DL;BROPHY, PJ
通讯作者：
BROPHY, PJ
影响因子：
9.2
作者：
Cox-Paulson, Elisabeth A.;Walck-Shannon, Elise;Lynch, Allison M.;Yamashiro, Sawako;Zaidel-Bar, Ronen;Eno, Celeste C.;Ono, Shoichiro;Hardin, Jeff
通讯作者：
Hardin, Jeff
影响因子：
3.7
作者：
Gokhin DS;Nowak RB;Kim NE;Arnett EE;Chen AC;Sah RL;Clark JI;Fowler VM
通讯作者：
Fowler VM
影响因子：
64.5
作者：
HIRANO, S;KIMOTO, N;TAKEICHI, M
通讯作者：
TAKEICHI, M
DOI：
10.1073/pnas.0808987105
发表时间：
2008-10-28
影响因子：
11.1
作者：
Cooper, Margaret A.;Son, Alexander I.;Zhou, Renping
通讯作者：
Zhou, Renping