Emerging links between surface nanotechnology and endocytosis: impact on nonviral gene delivery.
Emerging links between surface nanotechnology and endocytosis: impact on nonviral gene delivery.
复制标题
表面纳米技术与内吞作用之间的新兴联系：对非病毒基因递送的影响。
DOI：
10.1016/j.nantod.2010.10.007
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发表时间：
2010-12-01
期刊：
影响因子：
17.4
通讯作者：
Leong KW
中科院分区：
文献类型：
--
作者：
Adler AF;Leong KW
在改进非病毒载体介导的转染法方面继续付出了巨大的努力。这些努力通常集中在颗粒载体的设计上，这些颗粒载体具有鼓励在膜表面有效积累、颗粒摄取和内体逃逸的特性。尽管它在成功的非病毒感染中起着重要的作用，但对于驱动内化载体进入有利的非降解内吞途径的压力的研究相对较少。已经注意到底物介导法传递的复合体在转染率方面的改善，但这种增强背后的原因尚不清楚。细胞与纳米和微特征底物相互作用所表现出的表型变化提供了可能解释这些效应的线索。这篇综述描述了影响非病毒基因载体摄取和相互作用细胞的胞内表型的纳米颗粒和底物参数，并探索了可能介导这些相互作用的分子联系。底物介导的内吞作用控制代表了一个令人兴奋的新设计参数，将指导创造有效的转基因载体。
Significant effort continues to be exerted toward the improvement of transfection mediated by nonviral vectors. These endeavors are often focused on the design of particulate carriers with properties that encourage efficient accumulation at the membrane surface, particle uptake, and endosomal escape. Despite its demonstrated importance in successful nonviral transfection, relatively little investigation has been done to understand the pressures driving internalized vectors into favorable nondegradative endocytic pathways. Improvements in transfection efficiency have been noted for complexes delivered with a substrate-mediated approach, but the reasons behind such enhancements remain unclear. The phenotypic changes exhibited by cells interacting with nano- and micro-featured substrates offer hints that may explain these effects. This review describes nanoscale particulate and substrate parameters that influence both the uptake of nonviral gene carriers and the endocytic phenotype of interacting cells, and explores the molecular links that may mediate these interactions. Substrate-mediated control of endocytosis represents an exciting new design parameter that will guide the creation of efficient transgene carriers.
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