Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets
Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets
复制标题
宫内生长迟缓仔猪肠道中热休克蛋白 70 上调
DOI：
10.1007/s12192-009-0148-3
复制
发表时间：
2010-05
期刊：
影响因子：
--
通讯作者：
Li, Wei
中科院分区：
文献类型：
--
作者：
Zhong, Xiang;Wang, Tian;Zhang, Xuhui;Li, Wei
本研究旨在探讨热休克蛋白70（Hsp 70）在宫内生长迟缓（IUGR）仔猪肠道中的表达和分布。在出生时采集IUGR和正常体重（NBW）仔猪的十二指肠、空肠前、空肠远端、回肠和结肠样品。结果表明，IUGR仔猪的体重和肠重显著低于NBW仔猪。IUGR仔猪空回肠绒毛高度和绒毛/隐窝比显著低于NBW仔猪。提示IUGR可引起胃肠形态学异常和胃肠功能紊乱。IUGR仔猪空肠前（P < 0.05）、空肠远端（P <0.05）和结肠组织中hsp 70 mRNA的表达显著增加。IUGR仔猪回肠hsp 70 mRNA表达降低。IUGR仔猪空肠前（P < 0.05）、空肠远端（P < 0.05）和结肠（P < 0.05）中Hsp 70的表达水平高于NBW仔猪，与Hsp 70 mRNA表达水平相似。说明IUGR可上调仔猪肠道Hsp 70的表达，且不同肠道部位对应激的反应不同。同时，Hsp 70在整个绒毛和肠腺上皮细胞中的定位，而不是在固有层和肠肌中的定位，表明Hsp 70对上皮细胞的功能和结构具有细胞保护作用。
The objective of this study is to investigate the expression and distribution of heat shock protein 70 (Hsp70) in the intestine of intrauterine growth retardation (IUGR) piglets. Samples from the duodenum, prejejunum, distal jejunum, ileum, and colon of IUGR and normal-body-weight (NBW) piglets were collected at birth. The results indicated that the body and intestine weight of IUGR piglets were significantly lower than NBW piglets. The villus height and villus/crypt ratio in jejunum and ileum of IUGR piglets were significantly reduced compared to NBW piglets. These results indicated that IUGR causes abnormal gastrointestinal morphologies and gastrointestinal dysfunction. The mRNA of hsp70 was increased in prejejunum (P < 0.05), distal jejunum (P < 0.05), and colon in IUGR piglets. However, the hsp70 mRNA in ileum of piglets with IUGR was decreased. Similar to hsp70 mRNA, the protein levels of Hsp70 in prejejunum (P < 0.05), distal jejunum, and colon (P < 0.05) in IUGR piglets were higher than those in NBW piglets. These results indicated that the expression of Hsp70 in the intestinal piglets was upregulated by IUGR, and different intestinal sites had different responses to stress. Meanwhile, the localization of Hsp70 in the epithelial cells of the whole villi and intestinal gland rather than in the lamina propria and myenteron suggested that Hsp70 has a cytoprotective role in epithelial cell function and structure.
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DOI：
10.1016/b978-0-323-06545-0.00023-6
发表时间：
2011
期刊：
--
影响因子：
--
作者：
R. Kliegman
通讯作者：
R. Kliegman
影响因子：
2.5
作者：
Kojima, M;Hoshimaru, M;Kikuchi, H
通讯作者：
Kikuchi, H
热休克蛋白60在转运仔猪组织中的表达
影响因子：
3.8
作者：
Zhu, Lin;Bao, Endong;Hartung, Joerg
通讯作者：
Hartung, Joerg
DOI：
10.1016/b978-0-443-06930-7.50031-1
发表时间：
2001-01
影响因子：
3.8
作者：
通讯作者：
--
DOI：
--
发表时间：
1992-10
期刊：
Journal of developmental physiology
影响因子：
--
作者：
R. Xu;D. Mellor;P. Tungthanathanich;M. Birtles;G. Reynolds;H. Simpson
通讯作者：
R. Xu;D. Mellor;P. Tungthanathanich;M. Birtles;G. Reynolds;H. Simpson