Comparison of MY09/11 consensus PCR and type-specific PCRs in the detection of oncogenic HPV types.
Comparison of MY09/11 consensus PCR and type-specific PCRs in the detection of oncogenic HPV types.
复制标题
在检测致癌性HPV类型中，MY09/11共有PCR和类型特异性PCR的比较。
DOI：
10.1111/j.1582-4934.2007.00073.x
复制
发表时间：
2007-07
影响因子：
5.3
通讯作者：
Bogers JJ
中科院分区：
文献类型：
--
作者：
Depuydt CE;Boulet GA;Horvath CA;Benoy IH;Vereecken AJ;Bogers JJ
持续感染高危HPV和宫颈癌之间的因果关系导致了HPV DNA检测系统的发展。广泛使用的MY09/11共识PCR靶向HPV L1基因中450bp的保守序列，因此可以扩增广泛的HPV类型。然而，这些共识引物的局限性是显而易见的，特别是在不同HPV类型的检测灵敏度的可变性方面。本研究比较了MY09/11 PCR与类型特异性PCR在检测致癌型HPV中的应用。研究人群包括15,774名患者。共识PCR检测不出522例（10.9%）型特异性PCR提示的HPV感染。一致性PCR失败与HPV类型有显著相关性。HPV 51型、68型和45型最常被遗漏。MY09/11 PCR遗漏的HPV感染的临床相关性反映在细胞学异常的病例比例和随访中，有104例（25.4%）CIN2+病例。MY09/11假阴性可能是由于敏感性差，MY09/11引物不匹配或L1靶点被HPV整合或DNA降解破坏的结果。此外，MY09/11 PCR对致癌hpv缺乏特异性。从敏感性（my09 - 11 PCR: 87.9%；型别特异性PCR: 98.3%）和特异性（my09 - 11 PCR: 38.7%；型别特异性PCR: 76.14%）以及对组织学证实的CIN2+的预测值来看，PCR系统的诊断准确性提示型别特异性PCR可作为一种可靠的筛查工具用于临床。
The causal relationship between persistent infection with high-risk HPV and cervical cancer has resulted in the development of HPV DNA detection systems. The widely used MY09/11 consensus PCR targets a 450bp conserved sequence in the HPV L1 gene, and can therefore amplify a broad spectrum of HPV types. However, limitations of these consensus primers are evident, particularly in regard to the variability in detection sensitivity among different HPV types. This study compared MY09/11 PCR with type-specific PCRs in the detection of oncogenic HPV types. The study population comprised 15, 774 patients. Consensus PCR failed to detect 522 (10.9%) HPV infections indicated by type-specific PCRs. A significant correlation between failure of consensus PCR and HPV type was found. HPV types 51, 68 and 45 were missed most frequently. The clinical relevance of the HPV infections missed by MY09/11 PCR was reflected in the fraction of cases with cytological abnormalities and in follow-up, showing 104 (25.4%) CIN2+ cases. The MY09/11 false negativity could be the result of poor sensitivity, mismatch of MY09/11 primers or disruption of L1 target by HPV integration or DNA degradation. Furthermore, MY09/11 PCR lacked specificity for oncogenic HPVs. Diagnostic accuracy of the PCR systems, in terms of sensitivity (MY09/11 PCR: 87.9%; type-specific PCRs: 98.3%) and specificity (MY09/11 PCR: 38.7%; type-specific PCRs: 76.14%), and predictive values for histologically confirmed CIN2+, suggest that type-specific PCRs could be used in a clinical setting as a reliable screening tool.
登录
查看更多内容
影响因子：
8.8
作者：
通讯作者：
--
影响因子：
8.8
作者：
Depuydt, CE;Vereecken, AJ;Salembier, GM;Vanbrabant, AS;Boels, LA;van Herck, E;Arbyn, M;Segers, K;Bogers, JJ
通讯作者：
Bogers, JJ
影响因子：
120.7
作者：
BAUER, HM;TING, Y;MANOS, MM
通讯作者：
MANOS, MM
影响因子：
6.4
作者：
Cheung, Jo L. K.;Lo, Keith W. K.;Chan, Paul K. S.
通讯作者：
Chan, Paul K. S.
影响因子：
9.4
作者：
Qu, WM;Jiang, G;Burk, RD
通讯作者：
Burk, RD