Thin-layer liquid-based cervical cytology and PCR for detecting and typing human papillomavirus DNA in Flemish women.
Thin-layer liquid-based cervical cytology and PCR for detecting and typing human papillomavirus DNA in Flemish women.
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DOI：
10.1038/sj.bjc.6600756
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发表时间：
2003-02-24
影响因子：
8.8
通讯作者：
Bogers, JJ
中科院分区：
文献类型：
--
作者：
Depuydt, CE;Vereecken, AJ;Salembier, GM;Vanbrabant, AS;Boels, LA;van Herck, E;Arbyn, M;Segers, K;Bogers, JJ
本研究的目的是记录的发生，并与不同的人乳头瘤病毒（HPV）类型的流行率与细胞学结果同时进行薄层制剂在一个大的人口佛兰德妇女。在1年内，69 290薄层制剂进行了解释，使用贝塞斯达分类系统。使用基于共有引物和类型特异性PCR的HPV检测算法结合液基细胞学，我们确定了14种不同致癌HPV类型（16，18，31，33，35，39，45，51，52，56，58，59，66和68）的发生和分布。对细胞学异常的样本和年龄匹配的随机选择的宫颈细胞学正常的对照组（n=1351）进行反射HPV检测。给出了14种不同高危型HPV的细胞学、年龄和患病率之间的相关性。随着细胞学异常的增加，高危HPV类型的优势显著增加。多种HPV类型的合并感染也随着细胞学异常而增加，在HSIL中最高（16.7%）。在弗兰德斯，HSIL最常与HPV 16、33、35、31、18和51型相关。采用薄层液基细胞学和PCR检测HPV，是可行的筛查大量妇女。
The objective of this study was to document the occurrence and to correlate the prevalence of different human papillomavirus (HPV) types with the cytological results on simultaneously performed thin-layer preparations in a large population of Flemish women. During 1 year, 69 290 thin-layer preparations were interpreted using the Bethesda classification system. Using an algorithm for HPV testing based on consensus primers and type-specific PCRs in combination with liquid-based cytology, we determined the occurrence and distribution of 14 different oncogenic HPV types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68). Reflex HPV testing was performed on cytologically abnormal samples and on an age matched randomly selected control group with normal cervical cytology (n=1351). Correlation between cytology, age and prevalence for the 14 different high-risk HPV types is given. There is a significant increase in predominance of high-risk HPV types, with increasing abnormal cytology. Coinfection with multiple HPV types also increased with cytological abnormalities, and was highest in HSIL (16.7%). In Flanders, HSIL was most often associated with HPV types 16, 33, 35, 31, 18 and 51. Using thin-layer liquid-based cytology and PCR to detect HPV, it is feasible to screen large numbers of women.
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影响因子：
8.8
作者：
CUZICK, J;TERRY, G;ANDERSON, M
通讯作者：
ANDERSON, M
影响因子：
168.9
作者：
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120.7
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6.4
作者：
BERUMEN, J;CASAS, L;GARCIACARRANCA, A
通讯作者：
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