Biomass traits and candidate genes for bioenergy revealed through association genetics in coppiced European Populus nigra (L.).
Biomass traits and candidate genes for bioenergy revealed through association genetics in coppiced European Populus nigra (L.).
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DOI：
10.1186/s13068-016-0603-1
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发表时间：
2016
影响因子：
6.3
通讯作者：
Taylor G
中科院分区：
文献类型：
--
作者：
Allwright MR;Payne A;Emiliani G;Milner S;Viger M;Rouse F;Keurentjes JJ;Bérard A;Wildhagen H;Faivre-Rampant P;Polle A;Morgante M;Taylor G
来自木质纤维原料的第二代(2G)生物能源有潜力发展为一种可持续的可再生能源；然而，大规模商业化仍然存在重大障碍。杨树被认为是一种很有前途的2G原料，了解生物量产量和原料质量的遗传基础是该模式树种的研究重点。我们首次报道了欧洲原产树种欧洲黑杨广泛种群的714个成员的生物量研究，这些成员是从纬度和经度分别在40.5°和52.1°N和1.0°和16.4°E之间的20个河流种群中挑选出来的。当在英国南部的一个单一地点种植时，直接测量或衍生的15个性状中有14个具有显著的原产地(SO)效应，包括生物量产量、叶面积和气孔指数。在收割3年以上的生物产量性状之间存在显著的相关性(p<0.001)，叶大小和细胞产量是生物产量的有力预测因子。12个K的Illumina基因分型阵列(由14个QTL区域的10,331个SNPs和4,648个基因组成)突出了显著的群体遗传结构，成对的Fst显示出西班牙和意大利亚群体之间的强烈分化(p<0.001)。据报道，主茎高度和每叶细胞数这两个与生物量密切相关的性状达到了全基因组意义上的强健关联。这些基因分型和表型数据也被用来显示在该人群中存在显著的按距离隔离(IBD)和按适应隔离(IBA)。这三个被确认达到全基因组意义的关联包括转录因子、假定的压力反应基因和未知功能的基因。它们中没有一个以前与生物能源产量有关；在收集的三个选定基因类型的小组中显示出差异表达，并代表了在原产于欧洲和欧亚的生物能源树上进行进一步研究的令人兴奋的新候选者。另有26个标记(22个基因)达到推测意义，并对生物量、叶面积、表皮细胞扩展和气孔模式感兴趣。对欧洲黑杨的研究为开发利用生物能源的商品乡土树种和这些物种的先进分子育种提供了重要基础。本文的在线版本(doi：10.1186/s130680160603-1)包含补充材料，授权用户可以使用。
Second generation (2G) bioenergy from lignocellulosic feedstocks has the potential to develop as a sustainable source of renewable energy; however, significant hurdles still remain for large-scale commercialisation. Populus is considered as a promising 2G feedstock and understanding the genetic basis of biomass yield and feedstock quality are a research priority in this model tree species. We report the first coppiced biomass study for 714 members of a wide population of European black poplar (Populus nigra L.), a native European tree, selected from 20 river populations ranging in latitude and longitude between 40.5 and 52.1°N and 1.0 and 16.4°E, respectively. When grown at a single site in southern UK, significant Site of Origin (SO) effects were seen for 14 of the 15 directly measured or derived traits including biomass yield, leaf area and stomatal index. There was significant correlation (p < 0.001) between biomass yield traits over 3 years of harvest which identified leaf size and cell production as strong predictors of biomass yield. A 12 K Illumina genotyping array (constructed from 10,331 SNPs in 14 QTL regions and 4648 genes) highlighted significant population genetic structure with pairwise FST showing strong differentiation (p < 0.001) between the Spanish and Italian subpopulations. Robust associations reaching genome-wide significance are reported for main stem height and cell number per leaf; two traits tightly linked to biomass yield. These genotyping and phenotypic data were also used to show the presence of significant isolation by distance (IBD) and isolation by adaption (IBA) within this population. The three associations identified reaching genome-wide significance at p < 0.05 include a transcription factor; a putative stress response gene and a gene of unknown function. None of them have been previously linked to bioenergy yield; were shown to be differentially expressed in a panel of three selected genotypes from the collection and represent exciting, novel candidates for further study in a bioenergy tree native to Europe and Euro-Asia. A further 26 markers (22 genes) were found to reach putative significance and are also of interest for biomass yield, leaf area, epidermal cell expansion and stomatal patterning. This research on European P. nigra provides an important foundation for the development of commercial native trees for bioenergy and for advanced, molecular breeding in these species. The online version of this article (doi:10.1186/s13068-016-0603-1) contains supplementary material, which is available to authorized users.
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影响因子：
4.9
作者：
Arens, P;Coops, H;Vosman, B
通讯作者：
Vosman, B
尼格拉河群中的遗传和形态学分化：通过适应而隔离或隔离来隔离？
影响因子：
4.9
作者：
DeWoody J;Trewin H;Taylor G
通讯作者：
Taylor G
影响因子：
9.4
作者：
Cole, CT
通讯作者：
Cole, CT
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
7.2
作者：
Eloy, Nubia B.;Lima, Marcelo de Freitas;Ferreira, Paulo C. G.
通讯作者：
Ferreira, Paulo C. G.