Differential pro-inflammatory responses of astrocytes and microglia involve STAT3 activation in response to 1800 MHz radiofrequency fields.
Differential pro-inflammatory responses of astrocytes and microglia involve STAT3 activation in response to 1800 MHz radiofrequency fields.
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星形胶质细胞和小胶质细胞的差异促炎反应涉及响应 1800 MHz 射频场的 STAT3 激活
DOI：
10.1371/journal.pone.0108318
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhou Z
中科院分区：
文献类型：
--
作者：
Lu Y;He M;Zhang Y;Xu S;Zhang L;He Y;Chen C;Liu C;Pi H;Yu Z;Zhou Z
小胶质细胞和星形胶质细胞在维持中枢神经系统（CNS）内环境稳定中起重要作用。一些中枢神经系统的影响已被假定为与射频（RF）电磁场暴露。鉴于炎症在神经病理生理过程中的重要作用，我们研究了小胶质细胞和星形胶质细胞对射频场的促炎反应及其机制。将小胶质细胞N9和星形胶质细胞C8-D1 A细胞暴露于1800 MHz RF不同时间，用或不用STAT 3抑制剂预处理。小胶质细胞和星形胶质细胞被RF暴露激活，上调CD 11b和胶质细胞酸性蛋白（GFAP）。然而，RF暴露在星形胶质细胞和小胶质细胞中诱导了不同的促炎反应，其特征在于IL-1β、TNF-α、IL-6、PGE 2、一氧化氮（NO）、诱导型一氧化氮合酶（iNOS）和环氧合酶2（COX 2）的不同表达和释放谱。此外，RF暴露激活小胶质细胞中的STAT 3，而不是星形胶质细胞。此外，STAT 3抑制剂Stattic改善了小胶质细胞中RF诱导的促炎细胞因子的释放，但在星形胶质细胞中没有。我们的研究结果表明，RF暴露差异诱导小胶质细胞和星形胶质细胞的促炎反应，这涉及小胶质细胞和星形胶质细胞中STAT 3的差异激活。我们的数据提供了新的见解与移动的手机使用相关的CNS影响的潜在机制，目前STAT 3作为一个有前途的目标，以保护人类免受日益增加的RF暴露。
Microglia and astrocytes play important role in maintaining the homeostasis of central nervous system (CNS). Several CNS impacts have been postulated to be associated with radiofrequency (RF) electromagnetic fields exposure. Given the important role of inflammation in neural physiopathologic processes, we investigated the pro-inflammatory responses of microglia and astrocytes and the involved mechanism in response to RF fields. Microglial N9 and astroglial C8-D1A cells were exposed to 1800 MHz RF for different time with or without pretreatment with STAT3 inhibitor. Microglia and astrocytes were activated by RF exposure indicated by up-regulated CD11b and glial fibrillary acidic protein (GFAP). However, RF exposure induced differential pro-inflammatory responses in astrocytes and microglia, characterized by different expression and release profiles of IL-1β, TNF-α, IL-6, PGE2, nitric oxide (NO), inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2). Moreover, the RF exposure activated STAT3 in microglia but not in astrocytes. Furthermore, the STAT3 inhibitor Stattic ameliorated the RF-induced release of pro-inflammatory cytokines in microglia but not in astrocytes. Our results demonstrated that RF exposure differentially induced pro-inflammatory responses in microglia and astrocytes, which involved differential activation of STAT3 in microglia and astrocytes. Our data provide novel insights into the potential mechanisms of the reported CNS impacts associated with mobile phone use and present STAT3 as a promising target to protect humans against increasing RF exposure.
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影响因子：
5.1
作者：
Kang, Wenfei;Hebert, Jean M.
通讯作者：
Hebert, Jean M.
DOI：
10.1016/j.bbrc.2011.05.065
发表时间：
2011-06-17
影响因子：
3.1
作者：
Kim, Ki Chan;Joo, So Hyun;Shin, Chan Young
通讯作者：
Shin, Chan Young
影响因子：
1.9
作者：
Joubert, Vanessa;Leveque, Philippe;Yardin, Catherine
通讯作者：
Yardin, Catherine
影响因子：
1.9
作者：
Hirose, Hideki;Sasaki, Atsushi;Ugawa, Yoshikazu
通讯作者：
Ugawa, Yoshikazu
DOI：
10.1007/s00420-003-0446-5
发表时间：
2003-07-01
影响因子：
3
作者：
Ivancsits, S;Diem, E;Rüdiger, HW
通讯作者：
Rüdiger, HW