Smartphone-microfluidic fluorescence imaging system for studying islet physiology.
Smartphone-microfluidic fluorescence imaging system for studying islet physiology.
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DOI：
10.3389/fendo.2022.1039912
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发表时间：
2022
影响因子：
5.2
通讯作者：
Oberholzer, Jose
中科院分区：
文献类型：
--
作者：
Yu, Xiaoyu;Xing, Yuan;Zhang, Yiyu;Zhang, Pu;He, Yi;Ghamsari, Farid;Ramasubramanian, Melur K.;Wang, Yong;Ai, Huiwang;Oberholzer, Jose
智能手机技术最近已被应用于生物医学图像采集和数据分析，由于其高质量的成像能力，并灵活地定制多用途的应用程序。在这项工作中，我们开发并表征了一种用于研究胰岛生理学的智能手机微流体荧光成像系统。我们通过对用化学荧光染料或遗传编码荧光蛋白指示剂（GEFPI）标记的小鼠胰岛进行实时荧光成像来进一步评估系统能力。我们的研究结果表明，该系统能够分析关键的β细胞胰岛素刺激剂释放耦合因子响应于各种刺激与高分辨率的动力学。此外，微流体的集成允许在单个胰岛水平上高分辨率检测胰岛素分泌。与传统的荧光显微镜和宏观胰岛灌流仪相比，该系统具有成本低、携带方便、操作简单等优点。有了所有这些功能，我们设想这种智能手机-微流体荧光成像系统可以应用于研究胰岛生理学和临床应用。
Smartphone technology has been recently applied for biomedical image acquisition and data analysis due to its high-quality imaging capability, and flexibility to customize multi-purpose apps. In this work, we developed and characterized a smartphone-microfluidic fluorescence imaging system for studying the physiology of pancreatic islets. We further evaluated the system capability by performing real-time fluorescence imaging on mouse islets labeled with either chemical fluorescence dyes or genetically encoded fluorescent protein indicators (GEFPIs). Our results showed that the system was capable of analyzing key beta-cell insulin stimulator-release coupling factors in response to various stimuli with high-resolution dynamics. Furthermore, the integration of a microfluidics allowed high-resolution detection of insulin secretion at single islet level. When compared to conventional fluorescence microscopes and macro islet perifusion apparatus, the system has the advantages of low cost, portable, and easy to operate. With all of these features, we envision that this smartphone-microfluidic fluorescence imaging system can be applied to study islet physiology and clinical applications.
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一个基于微流体悬挂的胰岛渗漏系统，用于研究多个单个胰岛的葡萄糖刺激的胰岛素分泌。
影响因子：
5.7
作者：
Wu Jin P;Rousset N;Hierlemann A;Misun PM
通讯作者：
Misun PM
影响因子：
2.2
作者：
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通讯作者：
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影响因子：
3.9
作者：
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Westerlund, Fredrik
影响因子：
6.1
作者：
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通讯作者：
Huang, Tony Jun
影响因子：
6.2
作者：
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通讯作者：
Kerr-Conte, Julie