Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in Vitro Model.
Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in Vitro Model.
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DOI：
10.3390/ijms17101603
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发表时间：
2016-09-22
影响因子：
5.6
通讯作者：
Gurunathan S
中科院分区：
文献类型：
--
作者：
Zhang XF;Shen W;Gurunathan S
银纳米粒子(AgNPs)由于其独特的物理化学性质，特别是作为抗菌和抗癌药物，目前已被广泛应用于医药、化妆品、纺织、电子、医药等行业。最近，一些研究报道了AgNPs对各种原核和真核系统的有益和毒性作用。为了开发用于介导性治疗的纳米粒子，几个实验室在体外条件下使用了各种细胞系来评估AgNPs的性质、作用模式、差异反应和作用机制。体外模型简单、经济、快速，可以很容易地用于评估疗效和性能。AgNPs的细胞毒性、遗传毒性和生物相容性取决于许多因素，如大小、形状、表面电荷、表面涂层、溶解度、浓度、表面功能化、颗粒分布、进入方式、作用方式、生长介质、暴露时间和细胞类型。每种细胞类型对AgNPs的反应是不同的，取决于AgNPs的物理和化学性质。本综述评价了对AgNPs生物学应用文献的重大贡献。它从介绍AgNPs开始，特别注意它们对细胞效应的总体影响。这篇综述的主要目的是阐明不同类型的细胞对纳米颗粒表现出不同反应的原因，即使它们具有相似的大小、形状和其他参数。首先，我们讨论了AgNPs对多种细胞株的作用；其次，我们讨论了AgNPs在各种细胞系统中的作用机制，并试图阐明AgNPs是如何与不同的哺乳动物细胞株相互作用并产生显著影响的；最后，我们讨论了AgNPs对细胞的各种信号分子的激活，并对AgNPs的未来研究进行了展望。
Silver nanoparticles (AgNPs) have attracted increased interest and are currently used in various industries including medicine, cosmetics, textiles, electronics, and pharmaceuticals, owing to their unique physical and chemical properties, particularly as antimicrobial and anticancer agents. Recently, several studies have reported both beneficial and toxic effects of AgNPs on various prokaryotic and eukaryotic systems. To develop nanoparticles for mediated therapy, several laboratories have used a variety of cell lines under in vitro conditions to evaluate the properties, mode of action, differential responses, and mechanisms of action of AgNPs. In vitro models are simple, cost-effective, rapid, and can be used to easily assess efficacy and performance. The cytotoxicity, genotoxicity, and biocompatibility of AgNPs depend on many factors such as size, shape, surface charge, surface coating, solubility, concentration, surface functionalization, distribution of particles, mode of entry, mode of action, growth media, exposure time, and cell type. Cellular responses to AgNPs are different in each cell type and depend on the physical and chemical nature of AgNPs. This review evaluates significant contributions to the literature on biological applications of AgNPs. It begins with an introduction to AgNPs, with particular attention to their overall impact on cellular effects. The main objective of this review is to elucidate the reasons for different cell types exhibiting differential responses to nanoparticles even when they possess similar size, shape, and other parameters. Firstly, we discuss the cellular effects of AgNPs on a variety of cell lines; Secondly, we discuss the mechanisms of action of AgNPs in various cellular systems, and try to elucidate how AgNPs interact with different mammalian cell lines and produce significant effects; Finally, we discuss the cellular activation of various signaling molecules in response to AgNPs, and conclude with future perspectives on research into AgNPs.
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