The ionic products of bioactive glass particle dissolution enhance periodontal ligament fibroblast osteocalcin expression and enhance early mineralized tissue development.
The ionic products of bioactive glass particle dissolution enhance periodontal ligament fibroblast osteocalcin expression and enhance early mineralized tissue development.
复制标题
生物活性玻璃颗粒溶解的离子产物增强了牙周韧带成纤维细胞骨钙素的表达，并增强了早期矿化组织的发育。
DOI：
10.1002/jbm.a.33102
复制
发表时间：
2011-08
期刊：
影响因子：
--
通讯作者：
Loomer PM
中科院分区：
文献类型：
--
作者：
Varanasi VG;Owyoung JB;Saiz E;Marshall SJ;Marshall GW;Loomer PM
本研究结果显示，人牙周膜成纤维细胞(HPDLF)暴露于生物活性玻璃的条件培养液中，I型胶原和骨钙素的表达增加，这可能促进早期矿化组织的发育。用市售生物玻璃™(45S5)和实验生物活性涂层玻璃(6P53-b)制成玻璃条件培养液，并与对照培养液进行比较。电感耦合等离子体质谱分析显示，45S5 GCM细胞内钙离子、PO43-、Si4+和Na+浓度升高，而6P53-b GCM细胞内镁离子、K+、钙离子、PO43-、Si4+和Na+浓度升高(与对照相比)。经45S5和6P53-bGCM诱导的hPDLF分化培养显示，1型胶原(COL1α1、COL1α2)、骨钙素和碱性磷酸酶基因表达增强。这些GCM还促进了骨钙素蛋白的表达。培养16天后，45S5和6P53-b GCM处理的细胞显示出深红色的茜素染色区域，表明它们各自的细胞外基质(ECM)内的钙离子增加，而对照组细胞没有。扫描电子显微镜分析显示，GCM处理组细胞外基质更发达，表现为多层组织分层和丰富的胶原纤维束形成，而对照组细胞则没有。扫描电子显微镜分析显示，45S5 GCM处理的培养物呈多角形结构，提示有帽状结构。这些结果表明，生物活性涂层玻璃在牙周骨缺损填充应用中具有成骨潜力。
This study resulted in enhanced collagen type 1 and osteocalcin expression in human periodontal ligament fibroblasts (hPDLF) when exposed to glass conditioned media from bioactive glasses that subsequently may promote early mineralized tissue development. Commercial Bioglass™ (45S5) and experimental bioactive coating glass (6P53-b), were used to make a glass conditioned media (GCM) for comparison to control medium. ICP-MS analysis showed increased concentrations of Ca2+, PO43–,Si4+, and Na+, for 45S5 GCM and Mg2+, K+, Ca2+, PO43–,Si4+, and Na+ for 6P53-b GCM (relative to control medium). Differentiating hPDLF cultures exposed to 45S5 and 6P53-b GCM showed enhanced expression of collagen type 1 (Col1α1, Col1α2), osteocalcin, and alkaline phosphatase gene expression. These GCM also enhanced osteocalcin protein expression. After 16 days of culture, 45S5 and 6P53-b GCM treated cells showed regions of deep red Alizarin staining, indicating increased Ca within their respective extracellular matrices (ECM), while control-treated cells did not. SEM analysis showed more developed ECM in GCM treated cultures, indicated by multiple tissue layering and abundant collagen fiber bundle formation, while control treated cells did not. SEM analysis showed polygonal structures suggestive of CaP in 45S5 GCM treated cultures. These results indicate the osteogenic potential of bioactive coating glass in periodontal bone defect filling applications.
登录
查看更多内容
影响因子：
--
作者：
Inanc, B;Elcin, AE;Elcin, YM
通讯作者：
Elcin, YM
影响因子：
4.3
作者：
SHAPOFF, CA;BOWERS, GM;YUKNA, A
通讯作者：
YUKNA, A
影响因子：
6.7
作者：
Zamet, JS;Darbar, UR;Newman, HN
通讯作者：
Newman, HN
影响因子：
9.7
作者：
Foppiano, Silvia;Marshall, Sally J.;Tomsia, Antoni P.
通讯作者：
Tomsia, Antoni P.
DOI：
10.1006/bbrc.2001.4694
发表时间：
2001-04-20
影响因子：
3.1
作者：
Park, JC;Kim, YB;Han, KY
通讯作者：
Han, KY