Near-infrared assessments of skeletal muscle oxidative capacity in persons with spinal cord injury.
Near-infrared assessments of skeletal muscle oxidative capacity in persons with spinal cord injury.
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DOI：
10.1007/s00421-013-2657-0
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发表时间：
2013-09
影响因子：
3
通讯作者：
McCully, Kevin K.
中科院分区：
文献类型：
--
作者：
Erickson, Melissa Lynn;Ryan, Terence E.;Young, Hui-Ju;McCully, Kevin K.
关键词：
脊髓损伤（SCI）后，骨骼肌体积减小，肌内脂肪增加，线粒体功能可能下降。线粒体功能降低与代谢疾病的发展有关。本研究的目的是使用近红外光谱（NIRS）测量SCI患者的线粒体功能。在9名健全（AB）和9名运动完全性SCI患者反复短时间动脉闭塞期间，用近红外光谱法测量股外侧肌的耗氧量。用两种方法评估骨骼肌氧化能力（Vmax）：（1）运动后氧消耗恢复的速率常数和（2）从渐进工作试验中推断的最大氧消耗。与AB参与者相比，SCI参与者的Vmax（由恢复动力学测试的速率常数（k）表示）较低（k：SCI：0.7 ± 0.3 vs. AB：1.9 ± 0.4 min-1; p < 0.001）。时间常数为SCI：91.9 ± 37.8与AB：33.6 ± 8.3秒。来自渐进工作测试的Vmax在组之间接近显著差异（SCI：5.1 ± 2.9对AB：9.8 ± 5.5%Hb-Mb/sec; p = 0.06）。Vmax的NIRS测量结果表明，与AB对照组相比，SCI参与者的Vmax不足50-60%，这与先前使用31 P-MRS和肌肉活检的研究一致。NIRS测量可以评估SCI患者以及其他潜在受伤/患病人群的线粒体能力。
After spinal cord injury (SCI) skeletal muscle decreases in size, increases in intramuscular fat, and has potential declines in mitochondrial function. Reduced mitochondrial function has been linked to the development of metabolic disease. The aim of this study was to measure mitochondrial function in persons with SCI using near-infrared spectroscopy (NIRS). Oxygen consumption of the vastus lateralis muscle was measured with NIRS during repeated short-duration arterial occlusions in nine able-bodied (AB) and nine persons with motor complete SCI. Skeletal muscle oxidative capacity (Vmax) was evaluated with two approaches: (1) rate constant of the recovery of oxygen consumption after exercise and (2) extrapolated maximum oxygen consumption from a progressive work test. Vmax as indicated by the rate constant (k) from the recovery kinetics test was lower in SCI compared to AB participants (k: SCI: 0.7 ± 0.3 vs. AB: 1.9 ± 0.4 min−1; p < 0.001). Time constants were SCI: 91.9 ± 37.8 vs. AB: 33.6 ± 8.3 sec. Vmax from the progressive work test approached a significant difference between groups (SCI: 5.1 ± 2.9 vs. AB: 9.8 ± 5.5%Hb-Mb/sec; p = 0.06). NIRS measurements of Vmax suggest a deficit of 50-60% in participants with SCI compared to AB controls, consistent with previous studies using 31P-MRS and muscle biopsies. NIRS measurements can assess mitochondrial capacity in people with SCI, and potentially other injured/diseased populations.
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影响因子：
--
作者：
CHANCE, B;DAIT, MT;HAGERMAN, F
通讯作者：
HAGERMAN, F
影响因子：
3.3
作者：
McCully, Kevin K.;Mulcahy, Tara K.;Zhao, Qun
通讯作者：
Zhao, Qun
影响因子：
15.9
作者：
Anderson, Ethan J.;Lustig, Mary E.;Neufer, P. Darrell
通讯作者：
Neufer, P. Darrell
影响因子：
2.2
作者：
Elder, CP;Apple, DF;Dudley, GA
通讯作者：
Dudley, GA
DOI：
10.1073/pnas.82.24.8384
发表时间：
1985-12-01
影响因子：
11.1
作者：
CHANCE, B;LEIGH, JS;SMITH, D
通讯作者：
SMITH, D