Variable neuronal participation in stereotypic motor programs.
Variable neuronal participation in stereotypic motor programs.
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DOI：
10.1371/journal.pone.0040579
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Frost WN
中科院分区：
文献类型：
--
作者：
Hill ES;Vasireddi SK;Bruno AM;Wang J;Frost WN
运动神经网络在多大程度上是构成节律行为的刚性硬连线，而不是流体和动态实体？运动神经网络的成员是随时间变化的，还是随运动程序的情节变化的？这些问题只能在能够在运动程序产生期间监测神经元网络的尖峰活动的系统中解决。我们使用大规模的电压敏感染料（VSD）成像，然后进行独立成分分析尖峰分选，以检查在何种程度上的神经元网络背后的逃避游泳行为的Tritonia diomedea是硬连线与流体从一个时刻到时刻的角度来看。我们发现，虽然大多数神经元都致力于游泳网络，但有一小部分但很重要的神经元以令人惊讶的可变方式参与。这些神经元加入游泳运动程序较晚，离开较早，只在运动程序的某些周期或跳过的周期中爆发。我们证实，这种变量的神经元参与是不是由于VSD的影响，通过发现这样的神经元细胞内记录在无染料的盐水。此外，这些神经元在网络中的参与程度从游泳情节到情节显着变化。这种不可靠的爆裂神经元的普遍性，证实了他们的存在，在其他两个软体动物物种，Tritonia festiva和Aaplasia californica的节律性逃逸网络。我们的观察结果支持这样一种观点，即神经元网络，即使是那些潜在的节奏和刻板的运动程序，可能在结构上比广泛认识到的更多变。
To what extent are motor networks underlying rhythmic behaviors rigidly hard-wired versus fluid and dynamic entities? Do the members of motor networks change from moment-to-moment or from motor program episode-to-episode? These are questions that can only be addressed in systems where it is possible to monitor the spiking activity of networks of neurons during the production of motor programs. We used large-scale voltage-sensitive dye (VSD) imaging followed by Independent Component Analysis spike-sorting to examine the extent to which the neuronal network underlying the escape swim behavior of Tritonia diomedea is hard-wired versus fluid from a moment-to-moment perspective. We found that while most neurons were dedicated to the swim network, a small but significant proportion of neurons participated in a surprisingly variable manner. These neurons joined the swim motor program late, left early, burst only on some cycles or skipped cycles of the motor program. We confirmed that this variable neuronal participation was not due to effects of the VSD by finding such neurons with intracellular recording in dye-free saline. Further, these neurons markedly varied their level of participation in the network from swim episode-to-episode. The generality of such unreliably bursting neurons was confirmed by their presence in the rhythmic escape networks of two other molluscan species, Tritonia festiva and Aplysia californica. Our observations support a view that neuronal networks, even those underlying rhythmic and stereotyped motor programs, may be more variable in structure than widely appreciated.
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