Single-Cell Analysis for Glycogen Localization and Metabolism in Cultured Astrocytes
Single-Cell Analysis for Glycogen Localization and Metabolism in Cultured Astrocytes
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培养星形胶质细胞中糖原定位和代谢的单细胞分析
DOI：
10.1007/s10571-019-00775-4
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发表时间：
2019-12
影响因子：
4
通讯作者：
Shengxi Wu
中科院分区：
文献类型：
--
作者：
Yuanyuan Zhu;Ze Fan;Rui Wang;Rougang Xie;Haiyun Guo;Ming Zhang;Baolin Guo;Tangna Sun;Haifeng Zhang;Lixia Zhuo;Yan Li;Shengxi Wu
脑糖原主要位于星形胶质细胞而不是神经元中。糖原代谢与更高的脑功能有关，包括学习和记忆，但糖原在不同类型星形胶质细胞中的分布模式尚未完全描述。本研究采用基于2-NBDG掺入的方法研究了糖原在脑内的分布。我们确定了两种类型的星形胶质细胞，即2-NBDGI（糖原缺乏）和2-NBDGII（糖原丰富）细胞。采用全细胞膜片钳技术和荧光激活细胞分选技术分离2-NBDGI星形胶质细胞和2-NBDGI星形胶质细胞。分析2-NBDGI和2-NBDGI星形胶质细胞中糖原代谢酶的表达水平。我们发现2-NBDGI和2-NBDGI星形胶质细胞之间存在独特的糖原代谢模式。我们还观察到2-NBDG Ⅱ型星形胶质细胞主要是纤维型星形胶质细胞，而不是原生质型星形胶质细胞。我们的数据揭示了细胞类型依赖的糖原分布和代谢模式，表明这些不同的星形胶质细胞的不同功能。
Cerebral glycogen is principally localized in astrocytes rather than in neurons. Glycogen metabolism has been implicated in higher brain functions, including learning and memory, yet the distribution patterns of glycogen in different types of astrocytes have not been fully described. Here, we applied a method based on the incorporation of 2-NBDG, ad-glucose fluorescent derivative that can trace glycogen, to investigate glycogen’s distribution in the brain. We identified two types of astrocytes, namely, 2-NBDGI(glycogen-deficient) and 2-NBDGII(glycogen-rich) cells. Whole-cell patch-clamp and fluorescence-activated cell sorting (FACS) were used to separate 2-NBDGIIastrocytes from 2-NBDGIastrocytes. The expression levels of glycogen metabolic enzymes were analyzed in 2-NBDGIand 2-NBDGIIastrocytes. We found unique glycogen metabolic patterns between 2-NBDGIand 2-NBDGIIastrocytes. We also observed that 2-NBDGIIastrocytes were mainly identified as fibrous astrocytes but not protoplasmic astrocytes. Our data reveal cell type-dependent glycogen distribution and metabolism patterns, suggesting diverse functions of these different astrocytes.
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DOI：
10.1523/jneurosci.3605-10.2010
发表时间：
2010-10-20
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Sun D;Lye-Barthel M;Masland RH;Jakobs TC
通讯作者：
Jakobs TC
影响因子：
2.7
作者：
M. Raff;E. Abney;Rob Miller
通讯作者：
M. Raff;E. Abney;Rob Miller
影响因子：
3.9
作者：
T. Guillamón-Vivancos;U. Gómez-Pinedo;J. Matías‐Guiu
通讯作者：
T. Guillamón-Vivancos;U. Gómez-Pinedo;J. Matías‐Guiu
DOI：
10.1523/jneurosci.04-02-00585.1984
发表时间：
1984-02
期刊：
--
影响因子：
--
作者：
R. Miller;M. Raff
通讯作者：
R. Miller;M. Raff
影响因子：
3
作者：
Carmen Louzao, M.;Espina, Begona;Botana, Luis M.
通讯作者：
Botana, Luis M.