EPSP of L. casei BL23 Protected against the Infection Caused by Aeromonas veronii via Enhancement of Immune Response in Zebrafish.
EPSP of L. casei BL23 Protected against the Infection Caused by Aeromonas veronii via Enhancement of Immune Response in Zebrafish.
复制标题
DOI：
10.3389/fmicb.2017.02406
复制
发表时间：
2017
影响因子：
5.2
通讯作者：
Zhou Z
中科院分区：
文献类型：
--
作者：
Qin C;Zhang Z;Wang Y;Li S;Ran C;Hu J;Xie Y;Li W;Zhou Z
水产养殖是世界上增长最快的食品生产部门，供应了全球近50%的食用鱼供应。然而，疾病暴发已经成为养鱼业的一个主要问题。益生菌对水生动物健康的有益贡献已被广泛描述，并已被广泛应用于水产养殖中，用于疾病控制和促进生长。然而，益生菌成分的作用和益生菌提供的对病原体的保护机制仍然知之甚少。在本研究中，我们用17个潜在的益生菌菌株对斑马鱼幼体(孵化前)进行预定植，并筛选出具有抗Veronas气单胞菌感染作用的菌株。我们发现干酪乳杆菌BL23能显著提高斑马鱼幼体对白纹伊蚊感染的存活率。利用无菌斑马鱼模型和肠道微生物区系移植实验，证明干酪乳杆菌BL23本身对斑马鱼幼体具有抗感染作用，且不涉及微生物区系。此外，我们还鉴定了从干酪乳杆菌BL23细胞中提取的胞外多糖-蛋白复合体，它由一个40-45kD的蛋白质和一个由α-Rha、α-Glc、β-GlcNAc和β-GalNAc组成的胞外多糖组成。10~20μg/mlEPSP能显著提高斑马鱼感染白纹伊蚊后的存活率(P<0.01)。此外，EPSP还可诱导斑马鱼肝脏(ZFL)细胞TLR1和TLR2的高表达，并调节促炎和抗炎细胞因子的表达。我们的研究结果表明，干酪乳杆菌BL23来源的EPSP的抗感染作用是通过增强斑马鱼的免疫应答来实现的，这可能与TLR1/TLR2信号通路有关。
Aquaculture is the fastest-growing food production sector in the world, and it supplies nearly 50% of the global food fish supply. However, disease outbreaks have become a major problem in the fish farming industry. The beneficial contribution of probiotic bacteria to aquatic animals’ health has been widely described, and they have been widely used in aquaculture for disease control and growth promotion. However, the action of probiotic bacterial components and mechanisms underlying protection against pathogens afforded by probiotic bacteria remain poorly understood. In the present study, we pre-colonized zebrafish larvae (before hatching) with 17 potential probiotic bacterial strains and screened for those possessing anti-infective effects against Aeromonas veronii. We found that Lactobacillus casei BL23 significantly increased the survival of zebrafish larvae upon A. veronii infection. Using a germ-free (GF) zebrafish model and gut microbiota transplant experiment, we showed that L. casei BL23 per se has anti-infective effects in zebrafish larvae, which does not involve microbiota. Furthermore, we identified an exopolysaccharide-protein complex (EPSP) extracted from L. casei BL23 cells, which consisted of a 40–45 KD size protein and an exopolysaccharide composed of α-Rha, α-Glc, β-GlcNAc, and β-GalNAc. EPSP significantly increased the survival rate of GF zebrafish at a dose of 10–20 μg/ml after A. veronii infection (P < 0.01). In addition, the EPSP induced a higher expression of TLR1 and TLR2, and modulated the expression profile of pro-inflammatory and anti-inflammatory cytokines in zebrafish liver (ZFL) cells. Our data indicated that the anti-infective effect of EPSP from L. casei BL23 was mediated by enhancement of immune responses in zebrafish, which might involve the TLR1/TLR2 signal pathway.
登录
查看更多内容
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
1.2
作者：
de la Fuente, Mery;Miranda, Claudio D.;Urrutia, Homero
通讯作者：
Urrutia, Homero
影响因子：
5.2
作者：
Huang Y;Zhang L;Tiu L;Wang HH
通讯作者：
Wang HH
影响因子：
4.2
作者：
Kalliomaki, Marko;Antoine, Jean-Michel;Mercenier, Annick
通讯作者：
Mercenier, Annick
DOI：
10.1016/j.bbrc.2008.12.053
发表时间：
2009-02-06
影响因子：
3.1
作者：
Kim, Younghoon;Oh, Sejong;Kim, Sae Hun
通讯作者：
Kim, Sae Hun