Substance P-expressing excitatory interneurons in the mouse superficial dorsal horn provide a propriospinal input to the lateral spinal nucleus.
Substance P-expressing excitatory interneurons in the mouse superficial dorsal horn provide a propriospinal input to the lateral spinal nucleus.
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DOI：
10.1007/s00429-018-1629-x
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发表时间：
2018-06
影响因子：
3.1
通讯作者：
Todd AJ
中科院分区：
文献类型：
--
作者：
Gutierrez-Mecinas M;Polgár E;Bell AM;Herau M;Todd AJ
脊髓的浅层背角（板层I和II）包含许多兴奋性和抑制性中间神经元，最近的研究表明，这些组中的每一个都可以分为几个神经化学上不同的群体。虽然人们早就知道，在这个区域的一些神经元有intersegmented（propriospinal）轴突的预测，有关于propriospinal细胞的数量和轴突的范围相互矛盾的报告。此外，很少有人知道propriospinal神经元的神经化学表型或其轴突的终止模式。在本研究中，我们表明，使用逆行追踪，在腰膨大的I-II层神经元的约三分之一的项目至少有五个节段颅。P物质表达的兴奋性神经元在这些细胞中过度表达，占本体脊髓神经元的三分之一。相反，抑制性中间神经元和兴奋性PKCγ神经元在逆行标记的细胞中均代表不足。通过结合病毒载体介导的铬依赖性顺行追踪与免疫细胞化学，我们提供的证据表明，脊髓外侧核（LSN），而不是浅背角，是属于propriospinal P物质表达神经元的轴突的主要目标。这些发现有助于解决早期研究之间的差异，并对LSN在疼痛机制中的作用产生影响。
The superficial dorsal horn (laminae I and II) of the spinal cord contains numerous excitatory and inhibitory interneurons, and recent studies have shown that each of these groups can be divided into several neurochemically distinct populations. Although it has long been known that some neurons in this region have intersegmental (propriospinal) axonal projections, there have been conflicting reports concerning the number of propriospinal cells and the extent of their axons. In addition, little is known about the neurochemical phenotype of propriospinal neurons or about the termination pattern of their axons. In the present study we show, using retrograde tracing, that around a third of lamina I–II neurons in the lumbar enlargement project at least five segments cranially. Substance P-expressing excitatory neurons are over-represented among these cells, accounting for one-third of the propriospinal neurons. In contrast, inhibitory interneurons and excitatory PKCγ neurons are both under-represented among the retrogradely labelled cells. By combining viral vector-mediated Cre-dependent anterograde tracing with immunocytochemistry, we provide evidence that the lateral spinal nucleus (LSN), rather than the superficial dorsal horn, is the main target for axons belonging to propriospinal substance P-expressing neurons. These findings help to resolve the discrepancies between earlier studies and have implications for the role of the LSN in pain mechanisms.
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影响因子：
64.5
作者：
Duan B;Cheng L;Bourane S;Britz O;Padilla C;Garcia-Campmany L;Krashes M;Knowlton W;Velasquez T;Ren X;Ross S;Lowell BB;Wang Y;Goulding M;Ma Q
通讯作者：
Ma Q
影响因子：
7.4
作者：
Cameron D;Polgár E;Gutierrez-Mecinas M;Gomez-Lima M;Watanabe M;Todd AJ
通讯作者：
Todd AJ
影响因子：
5.5
作者：
Grudt, TJ;Perl, ER
通讯作者：
Perl, ER
影响因子：
2.9
作者：
GWYN D G;WALDRON H A
通讯作者：
WALDRON H A
影响因子：
3.3
作者：
HOKFELT, T;ELDE, R;ARIMURA, A
通讯作者：
ARIMURA, A