Regulation of LaSCL6 expression by genomic structure, alternative splicing, and microRNA in Larix kaempferi
Regulation of LaSCL6 expression by genomic structure, alternative splicing, and microRNA in Larix kaempferi
复制标题
落叶松中基因组结构、选择性剪接和 microRNA 对 LaSCL6 表达的调控
DOI：
10.1007/s11295-019-1362-5
复制
发表时间：
2019-06
影响因子：
2.4
通讯作者：
Li-Wang Qi
中科院分区：
文献类型：
--
作者：
Qiao-Lu Zang;Wan-Feng Li;Li-Wang Qi
SCR-LIKE-6（SCL 6）是GRAS转录因子家族的成员之一，在植物生长发育的许多方面起着重要作用。在先前的研究中，我们表明，转录后调控落叶松（La）SCL 6的microRNA miR 171功能在调节细胞分裂的模式和维持胚胎发生的潜力在落叶松体细胞胚胎发生。为了研究其在树木衰老过程中的表达模式，我们通过比较转录组学分析进行了研究，我们重新检查了每个转录本的注释和表达，发现了一个更长的转录本;我们将其注释为SCL 6，表明它可能是LaSCL 6的变体。为了验证这一点，我们克隆了该转录本的DNA和cDNA序列，发现在其表达中确实发生了选择性剪接。此外，这两种变体在胚性培养物和几个器官中均可检测到。值得注意的是，LaSCL 6的DNA序列包含简单的重复序列和单核苷酸多态性，这可能导致表达两种变体，并改变其三级结构。总之，LaSCL 6的表达受多个水平的调控，本研究不仅为LaSCL 6的表达提供了进一步的信息，也为研究基因表达调控提供了手段。
SCR-LIKE-6 (SCL6), a member of the GRAS transcription factor family, plays important roles in many aspects of plant growth and development. In a previous study, we showed that the post-transcriptional regulation of Larix (La)SCL6 by the microRNA miR171 functions in regulating the mode of cell division and the maintenance of embryogenic potential during somatic embryogenesis in larch. To investigate its expression pattern during tree aging, which has been studied by comparative transcriptomic analysis, we re-examined the annotation and expression of every transcript and found one that was longer; we annotated it as SCL6, indicating that it might be a variant of LaSCL6. To verify this, we cloned the DNA and cDNA sequences of this transcript and found that alternative splicing indeed occurred in its expression. Moreover, both variants were detectable in embryogenic cultures and several organs. Notably, the DNA sequence of LaSCL6 contained simple sequence repeats and a single-nucleotide polymorphism which might result in the expression of two variants with a change in their tertiary structures. Regulation of LaSCL6 by miR171 was also found. Taken together, the expression of LaSCL6 is controlled at several levels, and this study not only provides further information about the expression of LaSCL6 but also offers a means to study the regulation of gene expression.
登录
查看更多内容
影响因子：
5.3
作者：
Curaba J;Talbot M;Li Z;Helliwell C
通讯作者：
Helliwell C
DOI：
10.1038/nrm.2017.27
发表时间：
2017-07
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Baralle FE;Giudice J
通讯作者：
Giudice J
高松 miR171 的表征和主要功能分析
影响因子：
1.5
作者：
Hai, B. Z.;Qiu, Z. B.;Li, Y. F.
通讯作者：
Li, Y. F.
影响因子：
3.7
作者：
Airoldi CA;McKay M;Davies B
通讯作者：
Davies B
落叶松中 microRNA 的全基因组鉴定以及体细胞胚胎发生过程中 11 种保守 microRNA 及其靶标的阶段特异性调节
影响因子：
4.3
作者：
Zhang, Junhong;Zhang, Shougong;Han, Suying;Wu, Tao;Li, Xinmin;Li, Wanfeng;Qi, Liwang
通讯作者：
Qi, Liwang