A clock regulatory module is required for salt tolerance and control of heading date in rice.
A clock regulatory module is required for salt tolerance and control of heading date in rice.
复制标题
水稻的耐盐性和抽穗期控制需要时钟调节模块。
DOI：
10.1111/pce.14167
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Wang Lei
中科院分区：
文献类型：
--
作者：
Wang Xiling;He Yuqing;Wei Hua;Wang Lei
生物钟在植物逆境响应和发育过渡阶段中起着多重作用。然而，潜在的分子机制和个体时钟组件，协调调节水稻的重要农艺性状，如耐盐性和抽穗期仍有待阐明。在此，我们鉴定了一个由OsELF 4a、OsELF 3 -1和OsLUX组成的水稻三元阻遏蛋白复合体，命名为OsEC 1。OsELF 4a与OsELF 3 -1和OsELF 3 -2在细胞核内发生物理相互作用，而OsELF 3 -1与OsLUX（一个含有Myb结构域的转录因子）发生强烈相互作用，OsELF 3 -2与OsLUX的相互作用较弱。表型分析表明，该复合体在抽穗期和耐盐性中起作用。OsEC 1功能缺失突变体在盐胁迫和抽穗期延迟下的存活率较低。转录组学分析与生物化学测定一起鉴定了GIGANTEA同源物OsGI作为OsEC 1的直接转录靶点。值得注意的是，由CRISPR/Cas9产生的osgi-101突变体是耐盐的，并且在长日照条件下表现出较早的抽穗期。总之，我们的研究结果表征了水稻中由OsEC 1抑制OsGI组成的转录模块，OsGI将生物钟与耐盐性和抽穗期控制联系起来。本文受版权保护。All rights reserved.
The circadian clock plays multiple roles in plant stress responses and developmental transition phases. Nevertheless, the underlying molecular mechanisms and individual clock components that coordinately regulate important agronomic traits of rice such as salt tolerance and heading date remain to be elucidated. Here, we identify a rice ternary repressive protein complex composed of OsELF4a, OsELF3-1 and OsLUX, which was designated as OsEC1 in analogy to a similar complex in Arabidopsis. OsELF4a physically interacts with OsELF3-1 and OsELF3-2 in nucleus, while OsELF3-1 rather than OsELF3-2 strongly interacts with OsLUX, a Myb-domain containing transcriptional factor. Phenotypic analyses show a role for this complex in heading and salt tolerance. The loss-of-function mutants of OsEC1 exhibit lower survival rate under salt stress and late heading date. Transcriptomic profiling together with biochemical assays identified the GIGANTEA homologue OsGI as a direct transcriptional target of OsEC1. Notably, the osgi-101 mutant, generated by CRISPR/Cas9, is salt tolerant and exhibits early heading date in long day conditions. Together, our findings characterized a transcriptional module in rice composed by the OsEC1 repressing OsGI which links the circadian clock with salt tolerance and control of heading date. This article is protected by copyright. All rights reserved.
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DOI：
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发表时间：
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期刊：
The EMBO journal
影响因子：
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