Rlp7p is associated with 60S preribosomes, restricted to the granular component of the nucleolus, and required for pre-rRNA processing.
Rlp7p is associated with 60S preribosomes, restricted to the granular component of the nucleolus, and required for pre-rRNA processing.
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DOI：
10.1083/jcb.200111039
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发表时间：
2002-06-10
期刊：
影响因子：
--
通讯作者：
Hurt E
中科院分区：
文献类型：
--
作者：
Gadal O;Strauss D;Petfalski E;Gleizes PE;Gas N;Tollervey D;Hurt E
许多分析研究了真核细胞中的亚核仁结构，但形态结构，前rRNA加工和核糖体颗粒组装之间的关系仍然不清楚。使用用于输出60 S核糖体亚基的视觉测定，我们分离了ts-致死突变rix 9 -1，其导致Rpl 25 p-eGFP报告构建体的核仁积累。该突变导致Rlp 7 p中的单个氨基酸取代（F176 S），Rlp 7 p是与核糖体蛋白Rp 17 p相关的必需核仁蛋白。rix 9 -1（rlp 7 -1）突变阻断了ITS 2中C2位点的晚期前体RNA切割，该切割将前体分离为5.8S和25 S rRNA。与此一致，在非允许温度下，rlp 7 -1菌株中成熟的5.8S和25 S rRNA的合成被阻断，而18 S rRNA的合成继续进行。此外，原位杂交显示，含有ITS 2的pre-rRNA在rix 9 -1细胞的核仁中积累。最后，标记的Rlp 7 p被证明与前60 S颗粒，荧光显微镜和免疫EM本地化Rlp 7 p的核仁，这可能是颗粒组分（GC）的一个亚区。总之，这些数据表明，前rRNA切割位点C2特异性地需要Rlp 7 p，并发生在位于核仁GC区域的前60 S颗粒内。
Many analyses have examined subnucleolar structures in eukaryotic cells, but the relationship between morphological structures, pre-rRNA processing, and ribosomal particle assembly has remained unclear. Using a visual assay for export of the 60S ribosomal subunit, we isolated a ts-lethal mutation, rix9-1, which causes nucleolar accumulation of an Rpl25p-eGFP reporter construct. The mutation results in a single amino acid substitution (F176S) in Rlp7p, an essential nucleolar protein related to ribosomal protein Rpl7p. The rix9-1 (rlp7-1) mutation blocks the late pre-RNA cleavage at site C2 in ITS2, which separates the precursors to the 5.8S and 25S rRNAs. Consistent with this, synthesis of the mature 5.8S and 25S rRNAs was blocked in the rlp7-1 strain at nonpermissive temperature, whereas 18S rRNA synthesis continued. Moreover, pre-rRNA containing ITS2 accumulates in the nucleolus of rix9-1 cells as revealed by in situ hybridization. Finally, tagged Rlp7p was shown to associate with a pre-60S particle, and fluorescence microscopy and immuno-EM localized Rlp7p to a subregion of the nucleolus, which could be the granular component (GC). All together, these data suggest that pre-rRNA cleavage at site C2 specifically requires Rlp7p and occurs within pre-60S particles located in the GC region of the nucleolus.
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影响因子：
64.5
作者：
HUGLE, B;SCHEER, U;FRANKE, WW
通讯作者：
FRANKE, WW
影响因子：
1.6
作者：
Léger-Silvestre, I;Trumtel, S;Gas, N
通讯作者：
Gas, N
影响因子：
5.3
作者：
Santos-Rosa, H;Moreno, H;Hurt, E
通讯作者：
Hurt, E
影响因子：
14.9
作者：
SHUAI, K;WARNER, JR
通讯作者：
WARNER, JR
DOI：
10.1083/jcb.200108142
发表时间：
2001-12-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gleizes PE;Noaillac-Depeyre J;Léger-Silvestre I;Teulières F;Dauxois JY;Pommet D;Azum-Gelade MC;Gas N
通讯作者：
Gas N