Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells
Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells
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金钗石斛Lindl 甘露葡聚糖的结构表征及其乙酰化衍生物对 PC-12 细胞神经纤维发生的诱导作用
DOI：
10.3390/polym9090399
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发表时间：
2017-08
期刊：
影响因子：
5
通讯作者：
Kan Ding
中科院分区：
文献类型：
--
作者：
Can Jin;Zhenyun Du;Liyan Lin;Lishuang Zhou;Saijuan Li;Qin Liu;Kan Ding
从金钗石斛（Dendrobium nobile Lindl.）使用化学和光谱分析的组合，包括甲基化分析，部分酸水解，傅里叶变换红外（FTIR）光谱，气相色谱（GC），GC-质谱（MS）和核磁共振（NMR）光谱对JCS 1进行结构表征。采用高效凝胶渗透色谱法（HPGPC）测定其分子量为2.3 × 104 Da。糖组成分析表明其由葡萄糖、甘露糖、木糖和阿拉伯糖组成，摩尔比为40.2：2.3：1.7：1.0。结构分析表明，JCS 1是一个由（1→4）-β-Manp和（1→4）-α-Glcp组成的主链，在（1→4）-α-Glcp残基的C-6处有分支的甘露葡聚糖。分支由T-α-Glcp、1，4-α-Xylp和T-α-Araf组成。体外生物活性测试表明，JCS 1的乙酰化衍生物YJCS 1可诱导PC-12细胞的神经突形成。这些结果表明，YJCS 1可能是一个有前途的生物活性多糖开发作为一种可能的预防和治疗神经退行性疾病的候选药物。
A water-soluble polysaccharide (JCS1) was isolated from the stems of Dendrobium nobile Lindl. JCS1 was structurally characterized using a combination of chemical and spectral analysis, including methylation analysis, partial acid hydrolysis, Fourier-transform infrared (FTIR) spectroscopy, gas chromatography (GC), GC-mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy. The molecular weight was estimated to be 2.3 × 104 Da using high-performance gel permeation chromatography (HPGPC). The sugar composition analysis indicated it was composed of glucose, mannose, xylose, and arabinose in a 40.2:2.3:1.7:1.0 molar ratio. The structure analysis showed that JCS1 was a mannoglucan with a backbone consisting of (1→4)-linked β-Manp and (1→4)-linked α-Glcp with branches at C-6 of (1→4)-linked α-Glcp residues. The branches were composed of T-α-Glcp, 1,4-α-Xylp, and T-α-Araf. In vitro bioactivity tests revealed that the acetylated derivative of JCS1, YJCS1, induced neuritogenesis of PC-12 cells. These results demonstrate that YJCS1 might be a promising bioactive polysaccharide for development as a drug candidate for the possible prevention and treatment of neurodegeneration diseases.
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