Establishment and characterization of conditionally immortalized cells from the mouse urogenital ridge.
Establishment and characterization of conditionally immortalized cells from the mouse urogenital ridge.
复制标题
小鼠泌尿生殖嵴条件永生化细胞的建立和表征。
作者：
B. Capel;J. Hawkins;Elizabeth Hirst;Dimitris Kioussis;Robin Lovell
从泌尿生殖嵴的细胞培养已经建立，以促进哺乳动物性别决定的调控和下游相互作用的研究。细胞已从携带温度敏感性SV40大T抗原的转基因小鼠中分离，并在持续培养中建立。在电子显微镜水平上对这些培养物中的细胞进行分析，揭示了多种细胞类型，与该发育时期体内发现的细胞类型相比。在这些实验过程中同时被分离的原始生殖细胞也能在培养物中存活。当细胞被胰蛋白酶消化并接种在细胞外基质（Matrigel）中时，外植体经历形态发生组织成分支索状结构。我们分析了单层培养过程中，在Matrigel中体外形态发生过程中，以及在复杂外植体的克隆系中，胎儿性腺的一些分子标记物的表达。该分析包括Sry，发现其在来自XY泌尿生殖嵴的一些培养物中表达，所述培养物已保持长达8个月。
Cell cultures from the urogenital ridge have been established to facilitate the study of the regulation and downstream interactions of Sry in mammalian sex determination. Cells have been explanted from transgenic mice carrying a temperature sensitive SV40 large T-antigen, and established in ongoing cultures. Analysis of the cells in these cultures at the electron microscope level reveals multiple cell types that compare to the cell types found in vivo during this period of development. Primordial germ cells, that are simultaneously explanted in the course of these experiments, also survive in culture. The explants undergo a morphogenetic organization into branching cord-like structures when cells are trypsinized and plated in extracellular matrix (Matrigel). We analyzed the expression of a number of molecular markers of the fetal gonad during monolayer culture, during in vitro morphogenesis in Matrigel, and in clonal lines derived from the complex explants. This analysis included Sry which is found to be expressed in some cultures from XY urogenital ridges that have been maintained for as long as 8 months.
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影响因子：
4.6
作者：
G. Ryan;V. Steele-Perkins;J. Morris;F. Rauscher;G. Dressler
通讯作者：
G. Ryan;V. Steele-Perkins;J. Morris;F. Rauscher;G. Dressler
影响因子：
--
作者：
Y. Ikeda;W. Shen;H. Ingraham;K. Parker
通讯作者：
Y. Ikeda;W. Shen;H. Ingraham;K. Parker
DOI：
10.1073/pnas.89.4.1179
发表时间：
1992-02-15
影响因子：
11.1
作者：
DRESSLER, GR;DOUGLASS, EC
通讯作者：
DOUGLASS, EC
影响因子：
10.5
作者：
PELLETIER, J;SCHALLING, M;HOUSMAN, D
通讯作者：
HOUSMAN, D
影响因子：
3.7
作者：
HOFMANN, MC;NARISAWA, S;MILLAN, JL
通讯作者：
MILLAN, JL