Antigen is required for the activation of effector activities, whereas interleukin 2 Is required for the maintenance of memory in ovalbumin-specific, CD8+ cytotoxic T lymphocytes.
Antigen is required for the activation of effector activities, whereas interleukin 2 Is required for the maintenance of memory in ovalbumin-specific, CD8+ cytotoxic T lymphocytes.
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DOI：
10.1084/jem.187.1.49
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发表时间：
1998-01-05
期刊：
影响因子：
--
通讯作者：
Kapp JA
中科院分区：
文献类型：
--
作者：
Ke Y;Ma H;Kapp JA
维持T细胞记忆的机制还不完全清楚。我们已经研究了抗原和白细胞介素（IL）-2在CD 8 + T细胞的生长和维持中的作用，使用对卵清蛋白（OVA）257-264特异性的溶细胞T细胞系由H-2Kb呈递。该细胞系不分泌IL-4或IL-2;因此，在不添加外源生长因子的情况下，用OVA转染的EL 4细胞系（E.G7-OVA）刺激不会诱导增殖。此外，该细胞系可通过每周添加经辐照的脾填充细胞和IL-2（有或没有E.G7-OVA）来连续维持。尽管IL-2诱导这些细胞毒性T淋巴细胞（CTL）增殖，但干扰素γ和肿瘤坏死因子α的产生需要用E.G7-OVA刺激CTL。无论CTL在有或没有抗原（Ag）的情况下维持，经E.G7-OVA刺激后的淋巴因子分泌动力学是相同的。此外，两种CTL系均在4小时内杀死E.G7-OVA靶细胞。因此，这些CTL的效应子功能被T细胞受体（TCR）占据快速诱导。与或不与Ag培养的CTL也作为记忆T细胞时，停放在未经照射，无卵清蛋白的同基因受体100天。在不存在OVA的情况下，前体频率在正常和β2-微球蛋白敲除受体的脾脏中相同，但在IL-2敲除小鼠中显著降低。在缺乏IL-2的情况下记忆的下降支持来自其他研究者的数据，表明细胞周期对维持CD 8 + T细胞记忆很重要。这些数据还表明，通过淋巴因子刺激OVA特异性CTL似乎比通过与I类分子复合的交叉反应性肽刺激TCR对维持记忆更重要。
The mechanisms that maintain memory in T cells are not completely understood. We have investigated the role of antigen and interleukin (IL)-2 in the growth and maintenance of CD8+ T cells using a cytolytic T cell line specific for ovalbumin (OVA)257-264 presented by H-2Kb. This line does not secrete IL-4 or IL-2; hence, stimulation with the OVA-transfected EL4 line (E.G7-OVA) does not induce proliferation without addition of exogenous growth factors. Furthermore, this line can be maintained continuously by weekly addition of irradiated, splenic filler cells and IL-2, with or without E.G7-OVA. Although IL-2 induced proliferation of these cytotoxic T lymphocytes (CTLs), production of interferon γ and tumor necrosis factor α required stimulation of the CTL with E.G7-OVA. The kinetics of lymphokine secretion after stimulation by E.G7-OVA were the same whether the CTL had been maintained with or without antigen (Ag). In addition, both CTL lines killed E.G7-OVA target cells within 4 h. Thus, the effector functions of these CTLs were rapidly induced by T cell receptor (TCR) occupancy. CTLs cultured with or without Ag also served as memory T cells when parked for 100 d in unirradiated, syngeneic recipients without OVA. In the absence of OVA, the precursor frequency was identical in spleens of normal and β2-microglobulin knockout recipients, but significantly less in IL-2 knockout mice. The decline of memory in the absence of IL-2 supports data from other investigators, suggesting that cell cycling is important to the maintenance of CD8+ T cell memory. These data also suggest that stimulation of OVA-specific CTLs by lymphokines seems to be more important to maintaining memory than stimulation of TCRs by cross-reactive peptides complexed to class I molecules.
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影响因子：
5.4
作者：
ISAAZ, S;BAETZ, K;GRIFFITHS, GM
通讯作者：
GRIFFITHS, GM
影响因子：
64.8
作者：
LAU, LL;JAMIESON, BD;AHMED, R
通讯作者：
AHMED, R
影响因子：
56.9
作者：
CANTRELL, DA;SMITH, KA
通讯作者：
SMITH, KA
DOI：
10.1073/pnas.81.5.1509
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MEUER, SC;HUSSEY, RE;REINHERZ, EL
通讯作者：
REINHERZ, EL
DOI：
10.1084/jem.179.1.317
发表时间：
1994-01-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Müllbacher A
通讯作者：
Müllbacher A