Metaproteogenomic Profile of a Mesopelagic Adenylylsulfate Reductase: Course-Based Discovery Using the Ocean Protein Portal.
Metaproteogenomic Profile of a Mesopelagic Adenylylsulfate Reductase: Course-Based Discovery Using the Ocean Protein Portal.
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DOI：
10.1021/acs.jproteome.3c00152
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发表时间：
2023-09-01
影响因子：
4.4
通讯作者：
Saito, Mak A.
中科院分区：
文献类型：
--
作者：
Paoletti, Madeline M.;Fournier, Gregory P.;Dolan, Erin L.;Saito, Mak A.
腺苷硫酸盐还原酶(APR)是一种具有异化硫酸盐还原酶功能的黄素蛋白。其催化硫氧化剂中的逆反应的能力推动了与硫酸盐还原剂转移的复杂的系统发育历史，并使该酶成为海洋硫循环中的重要蛋白质。作为研究生课程的一部分，我们分析了来自海洋蛋白门户网站的Meta蛋白质组数据，并观察到中太平洋存在Aprα(APRA)和Beta(AprB)亚基的证据。该蛋白最初在分类学上被认为是一种绿色硫磺细菌Chlorobium tepidumTLS。然而，我们的系统基因组和海洋学背景分析与这个标签相矛盾，相反，我们表明这个蛋白质与新发现的念珠菌的基因组材料是一致的，这意味着这个谱系在氧气最少的黄昏地带的生态作用被低估。这项研究说明了元蛋白质组分析如何有助于更准确的元基因组/蛋白质组注释和有助于以课程为基础的研究经验的全面的海洋生物地球化学过程。
Adenylylsulfate reductase (Apr) is a flavoprotein with a dissimilatory sulfate reductase function. Its ability to catalyze the reverse reaction in sulfur oxidizers has propelled a complex phylogenetic history of transfers with sulfate reducers and made this enzyme an important protein in ocean sulfur cycling. As part of a graduate course, we analyzed metaproteomic data from the Ocean Protein Portal and observed evidence of Apr alpha (AprA) and beta (AprB) subunits in the Central Pacific Ocean. The protein was originally taxonomically attributed toChlorobium tepidumTLS, a green sulfur bacterium. However, our phylogenomic and oceanographic contextual analysis contradicted this label, instead showing that this protein is consistent with the genomic material from the newly discovered Candidatus Lambdaproteobacteriaclass, implying that the ecological role of this lineage in oxygen minimum twilight zones is underappreciated. This study illustrates how metaproteogenomic analysis can contribute to more accurate metagenomic/proteomic annotations and comprehensive ocean biogeochemical processes conducive to course-based research experiences.
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影响因子：
5.2
作者：
Pjevac P;Meier DV;Markert S;Hentschker C;Schweder T;Becher D;Gruber-Vodicka HR;Richter M;Bach W;Amann R;Meyerdierks A
通讯作者：
Meyerdierks A
影响因子：
2.9
作者：
Schiffer, A;Fritz, G;Ermler, U
通讯作者：
Ermler, U
影响因子：
4.4
作者：
Saito MA;Bertrand EM;Duffy ME;Gaylord DA;Held NA;Hervey WJ 4th;Hettich RL;Jagtap PD;Janech MG;Kinkade DB;Leary DH;McIlvin MR;Moore EK;Morris RM;Neely BA;Nunn BL;Saunders JK;Shepherd AI;Symmonds NI;Walsh DA
通讯作者：
Walsh DA
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
4.4
作者：
Saito MA;Saunders JK;Chagnon M;Gaylord DA;Shepherd A;Held NA;Dupont C;Symmonds N;York A;Charron M;Kinkade DB
通讯作者：
Kinkade DB