Trophoblasts promote induction of a regulatory phenotype in B cells that can protect against detrimental T cell-mediated inflammation.
Trophoblasts promote induction of a regulatory phenotype in B cells that can protect against detrimental T cell-mediated inflammation.
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DOI：
10.1111/aji.13187
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发表时间：
2019-12
期刊：
影响因子：
--
通讯作者：
Diener KR
中科院分区：
文献类型：
--
作者：
Guzman-Genuino RM;Dimova T;You Y;Aldo P;Hayball JD;Mor G;Diener KR
成功的妊娠结局关键取决于胚胎植入前母体免疫耐受的启动。与子宫微环境接触的胚胎来源的细胞可以对免疫细胞的表型和功能产生影响，以促进稳健的植入;然而，它们对B细胞的影响仍然未知。在这项研究中，我们研究了人滋养层细胞对B细胞表型的影响，以及随后对围着床事件的影响。我们培养纯化的人B细胞与早期妊娠人滋养层细胞系Swan 71，以研究滋养层-B细胞相互作用，并在迁移和侵袭的体外植入模型中利用滋养层球体。发现滋养层培养的B细胞或TE-B细胞由定向谱系中的B细胞如浆母细胞和记忆B细胞组成，以及CD 24 hiCD 27+调节B细胞和CD 19 +IL-10+ B细胞亚群的比例增加。来自TE-B细胞的条件培养基显示影响T细胞增殖和细胞因子产生的促炎细胞因子的产生减少。利用滋养层球体，我们评估了TE-B细胞在滋养层侵袭和迁移中的作用。我们的研究结果证明了TE-B条件培养基对有害炎症的保护作用，如在免疫攻击和促进迁移表型的存在下滋养层球体的存活所证明的。我们证实滋养层介导的B细胞的培养导致它们获得能够在围着床期调节子宫环境中炎症的特性。
A successful outcome to pregnancy is critically dependent on the initiation of maternal immune tolerance before embryo implantation. Cells of embryonic origin that come in contact with the uterine microenvironment can exert influence over the phenotype and function of immune cells to facilitate robust implantation; however, what influence they may have on B cells remains unknown. In this study, we investigate the effect of human trophoblast cells on B-cell phenotype and the subsequent effect on peri-implantation events. We cultured purified human B cells with the first-trimester human trophoblast cell line Swan 71 to investigate trophoblast-B-cell interactions and utilized trophoblast spheroids in an in vitro implantation model of migration and invasion. Trophoblast-educated B cells or TE-B cells were found to consist of B cells in committed lineages such as plasmablasts and memory B cells, as well as increased proportions in subsets of CD24hiCD27+ regulatory B cells and CD19+IL-10+ B cells. Conditioned media from the TE-B cells showed reduced production of pro-inflammatory cytokines that influenced the T-cell proliferation and cytokine production. Using trophoblast spheroids, we assessed the role of TE-B cells in trophoblast invasion and migration. Our results demonstrate a protective effect of TE-B-conditioned media against deleterious inflammation as evidenced by survival of the trophoblast spheroid in the presence of an immune assault and promotion of a migratory phenotype. We posit that trophoblast-mediated education of B cells leads to their acquisition of properties capable of modulating inflammation in the uterine environment during the peri-implantation period.
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影响因子：
5.2
作者：
Mor G;Cardenas I;Abrahams V;Guller S
通讯作者：
Guller S
影响因子：
4.6
作者：
Feyaerts D;Benner M;van Cranenbroek B;van der Heijden OWH;Joosten I;van der Molen RG
通讯作者：
van der Molen RG
影响因子：
8.6
作者：
Marie-Cardine, Aude;Divay, Florence;Jacquot, Serge
通讯作者：
Jacquot, Serge
影响因子：
7.3
作者：
Kaminski DA;Wei C;Qian Y;Rosenberg AF;Sanz I
通讯作者：
Sanz I
影响因子：
7.3
作者：
Guzman-Genuino RM;Diener KR
通讯作者：
Diener KR