Comparison of histochemical methods for murine eosinophil detection in an RSV vaccine-enhanced inflammation model.
Comparison of histochemical methods for murine eosinophil detection in an RSV vaccine-enhanced inflammation model.
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DOI：
10.1177/0192623308329342
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发表时间：
2009-02
影响因子：
1.5
通讯作者：
Varga SM
中科院分区：
文献类型：
--
作者：
Meyerholz DK;Griffin MA;Castilow EM;Varga SM
在固定的小鼠组织中，缺乏嗜酸性粒细胞组织化学检测的比较研究。在已建立的小鼠RSV疫苗增强炎症模型中，对先前报道的用于嗜酸性粒细胞检测的五种组织化学方法进行了定量和定性比较。在相应动物的嗜中性软组织病变和骨髓的组织切片中评价了非特异性中性粒细胞染色。嗜酸性粒细胞有颗粒状红色至橘红色的胞质染色，这取决于方法，而中性粒细胞，染色时，一个更均匀的胞质模式。在动脉壁和红细胞中观察到类似着色的非特异性背景染色。Astra Blue/Vital New Red、刚果红、Luna、改良苏木精-伊红和天狼星红技术均能有效检测到与对照组相比嗜酸性粒细胞募集增加;然而，不同技术之间嗜酸性粒细胞定量的差异显著不同。Astra Blue/Vital New Red对鉴别嗜酸性粒细胞和中性粒细胞的特异性最好，但计数嗜酸性粒细胞的能力降低，且时间最密集。Luna染色对组织的非特异性染色过多，浸润性嗜酸性粒细胞计数减少，使其不理想。对于多个参数，如嗜酸性粒细胞检测、特异性和与背景组织的对比度，天狼星红、刚果红和改良苏木精和伊红方法是有用的，每种方法都有自己的染色质量。
A comparative study of histochemical detection of eosinophils in fixed murine tissue is lacking. Five histochemical methods previously reported for eosinophil detection were quantitatively and qualitatively compared in an established murine RSV vaccine-enhanced inflammation model. Nonspecific neutrophil staining was evaluated in tissue sections of neutrophilic soft tissue lesions and bone marrow from respective animals. Eosinophils had granular red to orange-red cytoplasmic staining, depending on the method, whereas neutrophils had, when stained, a more homogenous cytoplasmic pattern. Nonspecific background staining of similar coloration was variably seen in arterial walls and erythrocytes. Astra Blue/Vital New Red, Congo Red, Luna, Modified Hematoxylin & Eosin, and Sirius Red techniques were all effective in detecting increased eosinophil recruitment compared to controls; however, differences in eosinophil quantification significantly varied between techniques. Astra Blue/Vital New Red had the best specificity for differentiating eosinophils and neutrophils, but had a reduced ability to enumerate eosinophils and was the most time intensive. The Luna stain had excessive non specific staining of tissues and a reduced enumeration of infiltrating eosinophils making it suboptimal. For multiple parameters such as eosinophil detection, specificity, and contrast with background tissues, the Sirius Red followed by Congo Red and Modified Hematoxylin & Eosin methods were useful, each with their own staining qualities.
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影响因子：
1.5
作者：
Arantes-Costa, Fernanda M.;Lopes, Fernanda D. T. Q. S.;Martins, Milton A.
通讯作者：
Martins, Milton A.
影响因子：
4.4
作者：
Vermaelen, KY;Cataldo, D;Pauwels, R
通讯作者：
Pauwels, R
影响因子：
5.4
作者：
Castilow, Elaine M.;Olson, Matthew R.;Vargal, Steven M.
通讯作者：
Vargal, Steven M.
影响因子：
1.1
作者：
DUFFY, JP;SMITH, PJ;MATTHEWS, HR
通讯作者：
MATTHEWS, HR
影响因子：
4.4
作者：
Lai, Wen-Qi;Goh, Hong Heng;Leung, Bernard P.
通讯作者：
Leung, Bernard P.