Decreasing microfluidic evaporation loss using the HMDL method: open systems for nucleic acid amplification and analysis
Decreasing microfluidic evaporation loss using the HMDL method: open systems for nucleic acid amplification and analysis
复制标题
使用 HMDL 方法减少微流体蒸发损失：用于核酸扩增和分析的开放系统
DOI：
10.1007/s10404-009-0508-4
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发表时间：
2010-07
影响因子：
2.8
通讯作者：
Zhang, Chunsun
中科院分区：
文献类型：
--
作者：
Xing, Da;Zhang, Chunsun
当处理具有开放的空气/液体界面的微流体装置时，由于大的表面积与体积比，蒸发是非常重要的。对于利用热反应（TR）储存器来执行一系列生物和化学反应的装置，过度的热诱导的微流体蒸发可快速导致反应储存器变干和整个装置的故障。在这项研究中，我们提出了一个简单的，新的方法来减少热诱导的流体蒸发微流体系统中，这是被称为热介导的扩散限制（HMDL）的方法。这种方法不需要复杂的热隔离来降低界面温度，也不需要连续向TR室中添加外部纯水来补偿蒸发损失。HMDL方法的原理是利用蒸发的反应内容物来增加扩散通道中的蒸气浓度。实验结果表明，基于HMDL方法的相对蒸发损失（Vloss/Vini）不仅取决于HMDL和TR区域的温度（THMDLandTTR），而且还取决于HMDL和TR的通道几何形状。采用直径为200 μm的U形均匀通道，在60 min内，Vloss/Vini低至5%（THMDL= 105°C，TTR= 95°C）。HMDL方法可用于设计用于核酸扩增和分析的开放式微流控系统，例如等温扩增和PCR热循环扩增，并且PCR过程已通过扩增来自单核细胞增生李斯特菌基因组DNA的135-bp片段而得到证实。
Evaporation is of great importance when dealing with microfluidic devices with open air/liquid interfaces due to the large surface-to-volume ratio. For devices utilizing a thermal reaction (TR) reservoir to perform a series of biological and chemical reactions, excessive heat-induced microfluidic evaporation can quickly lead to reaction reservoir dry out and failure of the overall device. In this study, we present a simple, novel method to decrease heat-induced fluid evaporation within microfluidic systems, which is termed as heat-mediated diffusion-limited (HMDL) method. This method does not need complicated thermal isolation to reduce the interfacial temperature, or external pure water to be added continuously to the TR chamber to compensate for evaporation loss. The principle of the HMDL method is to make use of the evaporated reaction content to increase the vapor concentration in the diffusion channel. The experimental results have shown that the relative evaporation loss (Vloss/Vini) based on the HMDL method is not only dependent on the HMDL and TR region’s temperatures (THMDLandTTR), but also on the HMDL and TR’s channel geometries. Using the U-shaped uniform channel with a diameter of 200 μm, theVloss/Viniwithin 60 min is low to 5% (THMDL= 105°C,TTR= 95°C). The HMDL method can be used to design open microfluidic systems for nucleic acid amplification and analysis such as isothermal amplification and PCR thermocycling amplification, and a PCR process has been demonstrated by amplifying a 135-bp fragment fromListeriamonocytogenesgenomic DNA.
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影响因子：
6.1
作者：
Walker, GM;Beebe, DJ
通讯作者：
Beebe, DJ
影响因子：
4.5
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Marcy, Yann;Ishoey, Thomas;Lasken, Roger S.;Stockwell, Timothy B.;Walenz, Brian P.;Halpern, Aaron L.;Beeson, Karen Y.;Goldberg, Susanne M. D.;Quake, Stephen R.
通讯作者：
Quake, Stephen R.
影响因子：
6.2
作者：
Litborn, E;Emmer, Å;Roeraade, J
通讯作者：
Roeraade, J
影响因子：
2.9
作者：
G. Ocvirk;Mark Munroe;T. Tang;R. Oleschuk;K. Westra;D. J. Harrison
通讯作者：
G. Ocvirk;Mark Munroe;T. Tang;R. Oleschuk;K. Westra;D. J. Harrison
影响因子：
2.8
作者：
Effenhauser, CS;Harttig, H;Krämer, P
通讯作者：
Krämer, P