Aquaculture production of hatchling Hawaiian Bobtail Squid (Euprymna scolopes) is negatively impacted by decreasing environmental microbiome diversity.
Aquaculture production of hatchling Hawaiian Bobtail Squid (Euprymna scolopes) is negatively impacted by decreasing environmental microbiome diversity.
复制标题
孵化夏威夷雪橇鱿鱼（Euprymna scolopes）的水产养殖产生受到降低环境微生物组多样性的负面影响。
DOI：
10.1111/jam.15350
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发表时间：
2022-03
影响因子：
4
通讯作者：
Hamilton-Brehm, Scott D.
中科院分区：
文献类型：
--
作者：
Murphy, Trevor R.;Xiao, Rui;Brooks, Marjorie L.;Rader, Bethany A.;Hamilton-Brehm, Scott D.
关键词：
夏威夷短尾鱿鱼（Euprymna scolopes）是研究宿主-共生体关系的模式生物。目前的科学重点是大肠杆菌内的微生物群，而对大肠杆菌储存罐内的微生物群知之甚少。我们研究了鱿鱼池环境中的细菌群落和地球化学与可存活的副鱿鱼生产相关的假设。从沉积物和过滤水样中提取总DNA，这些样品来自具有高胚胎存活率和卵旁孵化的“高产”鱿鱼群体，以及具有低胚胎存活率和卵旁孵化的“非高产”鱿鱼群体。作为对照，总DNA提取自海洋环境的地方，土着黄芪。采用下一代测序技术对细菌群落进行16S rRNA基因比对分析。在成虫水箱水体中鉴定出38个差异丰度属。大多数序列代表未分类、候选或新属。鉴定属包括Aquicella、Woeseia和Ferruginibacter，其中菌丝微生物和rhizohais在生产性成虫水箱水中更为丰富。此外，硝酸盐和pH值与生产队列共变，解释了67%的细菌种群。非生产性成人水箱水中硝酸盐还原细菌的丰度较低可以解释检测到的硝酸盐水平升高。我们得出结论，微生物组组成和水地球化学可能会对封闭水箱系统中镜蚶的生殖生理产生负面影响，最终影响利用这些动物进行的宿主微生物研究。这些结果表明，微生物组与地球化学之间存在着密切的关系。通过本研究，可以设计益生菌对抗措施来改善牛头螺杆菌的养殖条件。
The Hawaiian Bobtail Squid (Euprymna scolopes) is a model organism for investigating host–symbiont relationships. The current scientific focus is on the microbiome within E. scolopes, while very little is known about the microbiome of the tanks housing E. scolopes. We examined the hypothesis that bacterial communities and geochemistry within the squid tank environment correlate with the production of viable paralarval squid. Total DNA was extracted from sediment and filtered water samples from ‘productive’ squid cohorts with high embryonic survival and paralarval hatching, ‘unproductive’ cohorts with low embryonic survival and paralarval hatching. As a control total DNA was extracted from environmental marine locations where E. scolopes is indigenous. Comparative analysis of the bacterial communities by the 16S rRNA gene was performed using next generation sequencing. Thirty‐eight differentially abundant genera were identified in the adult tank waters. The majority of the sequences represented unclassified, candidate or novel genera. The characterized genera included Aquicella, Woeseia and Ferruginibacter, with Hyphomicrobium and Rhizohapis were found to be more abundant in productive adult tank water. In addition, nitrate and pH covaried with productive cohorts, explaining 67% of the bacterial populations. The lower abundance of nitrate‐reducing bacteria in unproductive adult tank water could explain detected elevated nitrate levels. We conclude that microbiome composition and water geochemistry can negatively affect E. scolopes reproductive physiology in closed tank systems, ultimately impacting host‐microbe research using these animals. These results identify the tight relationship between the microbiome and geochemistry to E. scolopes. From this study, it may be possible to design probiotic counter‐measures to improve aquaculture conditions for E. scolopes.
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影响因子：
4.6
作者：
Giatsis C;Sipkema D;Smidt H;Heilig H;Benvenuti G;Verreth J;Verdegem M
通讯作者：
Verdegem M
影响因子：
2.7
作者：
Pearman JK;Keeley NB;Wood SA;Laroche O;Zaiko A;Thomson-Laing G;Biessy L;Atalah J;Pochon X
通讯作者：
Pochon X
影响因子：
4.4
作者：
Collins, Andrew J.;LaBarre, Brenna A.;Nyholm, Spencer V.
通讯作者：
Nyholm, Spencer V.
影响因子：
4.5
作者：
Fossmark, Ragnhild O.;Vadstein, Olav;Attramadal, Kari J. K.
通讯作者：
Attramadal, Kari J. K.
影响因子：
6.7
作者：
Finney CA;Kamhawi S;Wasmuth JD
通讯作者：
Wasmuth JD